Transcriptome Analysis Reveals the Regulatory Mechanism of Lipid Metabolism and Oxidative Stress in Litopenaeus vannamei under Low-Salinity Stress

文献类型： 外文期刊

第一作者： Cao, Siyao

作者： Cao, Siyao;Li, Yundong;Jiang, Song;Yang, Qibin;Huang, Jianhua;Yang, Lishi;Shi, Jianzhi;Jiang, Shigui;Wen, Guoliang;Zhou, Falin;Cao, Siyao;Li, Yundong;Wen, Guoliang;Zhou, Falin;Cao, Siyao;Li, Yundong;Jiang, Song;Yang, Qibin;Huang, Jianhua;Yang, Lishi;Shi, Jianzhi;Jiang, Shigui;Wen, Guoliang;Zhou, Falin;Li, Yundong;Yang, Qibin;Huang, Jianhua;Zhou, Falin

作者机构：

关键词： Litopenaeus vannamei; salinity stress; transcriptome analysis; differentially expressed genes; peroxisome

期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.8； 五年影响因子：2.8 ）

ISSN：

年卷期： 2024 年 12 卷 8 期

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收录情况： SCI

摘要： Salinity is a crucial environmental factor influencing the survival, growth, development, and reproduction of aquatic animals. However, the underlying molecular mechanisms of the shrimp's response to salinity stress are not yet fully understood. Therefore, we used the Illumina NovaSeq 6000 platform to perform transcriptome sequencing of the hepatopancreas of Litopenaeus vannamei under high-salinity (30 PSU), medium-salinity (10 PSU), and low-salinity (0.5 PSU) conditions. We obtained 63.23 Gb of high-quality data and identified 3589 differentially expressed genes (DEGs), including 1638 upregulated and 1951 downregulated genes. Notably, a comparison between the control group (30 PSU) and the low-salinity group (0.5 PSU) revealed that the BBOX1 and CHE1 genes were significantly upregulated, while the ACOX1, MPV, CYP2L1, GCH, MVK, TREt1, and XDH genes were significantly downregulated. These genes are primarily involved in key metabolic pathways, such as fatty acid oxidation, cholesterol metabolism, and hormone synthesis and metabolism. The key genes involved in fatty acid beta-oxidation, such as ACOX1, ACAD, HADH, HSD17B4, PECR, CROT, PIPOX, and CG5009, all showed a downward trend, suggesting that L. vannamei may respond to salt stress by regulating fatty acid oxidative metabolism, optimizing energy utilization, and maintaining cell homeostasis under low-salinity conditions. Functional annotation of gene ontology (GO) and KEGG pathway enrichment analysis highlighted the roles of these significant DEGs in the adaptation of L. vannamei to environments of varying salinity, underscoring the importance of metabolic pathways in their adaptive physiological responses. This study provides a crucial molecular biological basis for understanding the molecular mechanisms and physiological protection strategies of L. vannamei under salinity stress.

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