Structure and chain conformation of bioactive beta-D-glucan purified from water extracts of Ganoderma lucidum unbroken spores

文献类型： 外文期刊

第一作者： Liu, Yanfang

作者： Liu, Yanfang;Wang, Yatao;Zhou, Shuai;Yan, Mengqiu;Tang, Qingjiu;Zhang, Jingsong

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关键词： Ganoderma lucidum unbroken spores; beta-D-glucan; Structural and conformational characteristic

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 180 卷

页码：

收录情况： SCI

摘要： Two polysaccharide fractions (GLSB50 and GLSB70) with total sugar content of 82.07 wt% and 53.79 wt%, respectively, were obtained from the water extracts of unbroken Ganoderma lucidum spores by sequential ethanol precipitation treatment. Compared with GLSB70. GLSB50 exhibited better activity on stimulation of humoral immune responses in immunosuppressed mice. A novel beta-D-glucan (GLSB50A-III-1) with weight average molecular weight (M-w) of 1.93 x 10(5) g/mol was purified from GLSB50 through chromatography separation. The exponent alpha value of Mark-Houwink-Sakurada equation was calculated to be 0.13, indicating that GLSB50A-III-1 presented globular spheres conformation in aqueous solution. Structural analysis showed that GLSB50A-III-1 mainly consisted of (1 -> 3), (1 -> 4), (1 -> 6)-linked beta-D-glucose residues in the backbone, with two single beta-D-Glcp attached at 0-6 of beta-(1 -> 3) and beta-(1 -> 4)-linked residues separately as side chains. The repeat unit of GISB50A-III-1 was deduced as follows: [GRAPHICS] . (C) 2021 Published by Elsevier B.V.

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