Beta-ionone is a functional plant volatile that attracts the parasitic wasp, Microplitis pallidipes

文献类型： 外文期刊

第一作者： Zhang, Hao

作者： Zhang, Hao;Wang, Jin-Yan;Chen, Yi-Juan;Ji, Xiang-Yun;Jiang, Jie-Xian;Wan, Nian-Feng;Siemann, Evan;Wan, Nian-Feng

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关键词： Biological control; Field screenhouse experiments; Fluorescence competitive binding assay; Odorant binding protein; Parasitoid; Y-tube olfactometer

期刊名称：BIOCONTROL （ 影响因子：3.571； 五年影响因子：3.243 ）

ISSN： 1386-6141

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收录情况： SCI

摘要： The effects of plant volatiles on parasitoids are important with regards to the tri-trophic interactions among host plants, insect herbivores, and their natural enemies. However, the effects of plant volatiles on the important parasitoid (Microplitis pallidipes Szepligeti; Hymenoptera: Braconidae) of noctuids have not been determined at the molecular, laboratory, and outdoor scales with complementary approaches. The odorant binding protein 8 of M. pallidipes (MpOBP8) with an open reading frame of 459 bp was cloned and successfully expressed in Escherichia coli (Eubacteriales: Enterobacteriaceae). The purified protein MpOBP8 had a relatively high binding capacity to the plant volatile beta-ionone (Ki value was 28.86 mu M) in the fluorescence competitive binding assay. Meanwhile, Y-tube olfactometer experiments indicated that beta-ionone selectively attracts the parasitic wasp as the ratio of M. pallidipes selecting beta-ionone vs. control was 2:1. Likewise, field screenhouse experiments verified the above results as the ratio of M. pallidipes selecting beta-ionone vs. control was close to 2:1. This study suggested that beta-ionone is a plant volatile that attracts the parasitoid M. pallidipes, and MpOBP8 may play a key role in the discrimination of M. pallidipes in searching for hosts via beta-ionone. In general our results suggest that beta-ionone has the potential to enhance the biocontrol effect of parasitoids on insect herbivores.

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