GmAP1d regulates flowering time under long-day photoperiods in soybean

文献类型： 外文期刊

第一作者： Guo, Shiyu

作者： Guo, Shiyu;Li, Xiaoyu;Li, Ying-hui;Guo, Shiyu;Li, Yanfei;Zhang, Hao;Tian, Yu;Li, Xiaoyu;Liu, Bin;Li, Ying-hui;Qiu, Li-juan;Qiu, Hongmei;Hu, Guoyu;Zhao, Chaosen;Wang, Ruizhen;Li, Yanfei

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关键词： Soybean; Flowering time; GWAS; GmAP1d; Long-day conditions

期刊名称：CROP JOURNAL （ 影响因子：6.0； 五年影响因子：5.6 ）

ISSN： 2095-5421

年卷期： 2024 年 12 卷 3 期

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收录情况： SCI

摘要： Flowering time is important for adaptation of soybean ( Glycine max ) to different environments. Here, we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions. We identified three strong signals at the qFT02-2 locus (Chr02: 12037319-12238569), which were associated with flowering time in three environments: Gongzhuling, Mengcheng, and Nanchang. By analyzing linkage disequilibrium, gene expression patterns, gene annotation, and the diversity of variants, we identified an AP1 homolog as the candidate gene for the qFT02-2 locus, which we named GmAP1d . Only one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:12087053. Accessions carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A allele. Thus, we developed a cleaved amplified polymorphic sequence (CAPS) marker for the SNP at Chr02:12087053, which is suitable for marker-assisted breeding of flowering time. Knockout of GmAP1d in the 'Williams 82' background by gene editing promoted flowering under long-day conditions, confirming that GmAP1d is the causal gene for qFT02-2 . An analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of soybean. Through stepwise selection, the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased, and this allele has become nearly fixed (95%) in northern China. These findings provide a theoretical basis for better understanding the molecular regulatory mechanism of flowering time in soybean and a target gene that can be used for breeding modern soybean cultivars adapted to different latitudes. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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