Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China

文献类型： 外文期刊

第一作者： Chen, Yuan

作者： Chen, Yuan;Hao, Kaiqiang;Wang, Zhiping;An, Mengnan;Xia, Zihao;Zhou, Xueping;Wu, Yuanhua;Guo, Shiping;Jiang, Lianqiang;Yan, Fangfang;Li, Fangfang;Zhou, Xueping;Zhou, Xueping

作者机构：

关键词： Geminivirus; Begomovirus; Tomato leaf curl chuxiong virus; Infectious clone; Pathogenicity

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2024 年 594 卷

页码：

收录情况： SCI

摘要： The occurrence of geminiviruses causes significant economic losses in many economically important crops. In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames. Phylogenetic and genome-wide pairwise identity analysis revealed that TLCCxV shared less than 91% identities with reported geminiviruses. A TLCCxV infectious clone was constructed and successfully infected Nicotiana benthamiana, N. tabacum, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants. Furthermore, expression of the V2, C1 and C4 proteins through a potato virus X vector caused severe chlorosis or necrosis symptom in N. benthamiana. Taken together, we identified a new geminivirus in tobacco plants, and found that V2, C1 and C4 contribute to symptom development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序