Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5
文献类型： 外文期刊
第一作者： Han, Jinze
作者： Han, Jinze;Niu, Xinxin;Ge, Chengfei;Wu, Ziwen;Wang, Guodong;Huang, Mengmeng;Zhang, Yulong;Liu, Runhang;Xu, Mengmeng;Yu, Hangbo;Han, Jingzhe;Wang, Suyan;Liu, Yongzhen;Chen, Yuntong;Cui, Hongyu;Zhang, Yanping;Duan, Yulu;Wang, Xiaomei;Gao, Yulong;Qi, Xiaole;Han, Jinze;Niu, Xinxin;Wu, Ziwen;Wang, Guodong;Huang, Mengmeng;Zhang, Yulong;Liu, Runhang;Xu, Mengmeng;Yu, Hangbo;Han, Jingzhe;Wang, Suyan;Cui, Hongyu;Wang, Xiaomei;Gao, Yulong;Qi, Xiaole;Han, Jinze;Xu, Mengmeng;Han, Jingzhe;Li, Liuan;Wang, Suyan;Liu, Yongzhen;Chen, Yuntong;Cui, Hongyu;Zhang, Yanping;Duan, Yulu;Wang, Xiaomei;Gao, Yulong;Qi, Xiaole
作者机构：
关键词： Infectious bursal disease virus; VP5; Soluble expression; Monoclonal antibody; Antigen epitope
期刊名称：VETERINARY JOURNAL （ 影响因子：3.1； 五年影响因子：2.7 ）
ISSN： 1090-0233
年卷期： 2024 年 308 卷
页码：
收录情况： SCI
摘要： Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.
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