Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis

文献类型： 外文期刊

第一作者： Chen, Wenqiang

作者： Chen, Wenqiang;Lin, Xiaodong;Wang, Yan;Mu, Detian;Tang, Qi;Mo, Changming;Huang, Huaxue;Liu, Dai;Zhao, Huan;Luo, Zuliang;Wilson, Iain W.;Qiu, Deyou

作者机构：

关键词： Siraitia siamensis; reference genes; RT-qPCR; mogrosides; synthesis pathways

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 17 期

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收录情况： SCI

摘要： Siraitia siamensis is a traditional Chinese medicinal herb. In this study, using S. siamensis cultivated in vitro, twelve candidate reference genes under various treatments were analyzed for their expression stability by using algorithms such as GeNorm, NormFinder, BestKeeper, Delta CT, and RefFinder. The selected reference genes were then used to characterize the gene expression of cucurbitadienol synthase, which is a rate-limiting enzyme for mogroside biosynthesis. The results showed that CDC6 and NCBP2 expression was the most stable across all treatments and are the best reference genes under the tested conditions. Utilizing the validated reference genes, we analyzed the expression profiles of genes related to the synthesis pathway of mogroside in S. siamensis in response to a range of abiotic stresses. The findings of this study provide clear standards for gene expression normalization in Siraitia plants and exploring the rationale behind differential gene expression related to mogroside synthesis pathways.

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