Competitive exclusion of insect parasitic fungi: Enhanced metabolite biosynthesis in Beauveria bassiana and fatty acid production in Metarhizium robertsii during host coinfection

文献类型： 外文期刊

第一作者： Liu, Kexin

作者： Liu, Kexin;Li, Gen;Wu, Siying;Zhang, Yang;Lv, Cheng;Yue, Xingcheng;Zhu, Zhuoran;Zhou, Tianzi;Huang, Bo;Wang, Yulong;Zhou, Rong

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关键词： Entomopathogenic fungi; Competition; In vivo; Fatty acid biosynthesis; 2-pyridone

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 214 卷

页码：

收录情况： SCI

摘要： Microbial consortia, involving two or more microorganisms, have been explored for pest management purposes, despite concerns regarding competitive exclusion among entomopathogenic fungi that may undermine synergistic effects. However, the precise molecular mechanisms governing entomopathogen competition in vivo remain inadequately elucidated. Here, we investigate competitive exclusion dynamics between two prominent entomopathogens, Metarhizium robertsii and Beauveria bassiana. Coinfection assays employing diverse strains of B. bassiana and M. robertsii, varying in virulence, reveal inter-specific competition leading to reduced pathogenicity to Galleria mellonella. Both fungi exhibit inhibited hindwing penetration efficiencies during coinfection, with M. robertsii demonstrating superior competitive ability within the insect host. Transcriptome analysis highlights the regulation of the MAPK signaling pathway by both fungi during host coinfection. In vivo, enhanced fatty acid biosynthesis is observed in M. robertsii, contrasting with the activation of 2-pyridone production in B. bassiana. In the insect hemocoels, overexpression of the fatty acid synthase gene (FAST) significantly increases M. robertsii ratios during mixed infections, whereas FAST silencing decreases these ratios. Conversely, minimal changes in M. robertsii ratios are observed following FAST expression modulation in artificial medium co-cultures. Our study confirms that inter-species competitive exclusion among insect parasitic fungi diminishes pathogenicity, underscore the intricate interactions between entomopathogenic fungi and emphasize the caution required when considering combined fungal parasite strategies for insect pest control.

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