Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits

文献类型： 外文期刊

第一作者： Song, Weiyuan

作者： Song, Weiyuan;Huang, Yuxiang;Cheng, Zhihua;Tian, Di;Geng, Yan;Guo, Jingyu;Li, Chuang;She, Daixi;Zhong, Yanting;Li, Min;Liu, Liu;Chen, Jiacai;Zhou, Zhaoyang;Zhao, Jianyu;Zhang, Xiaolan;Xie, Yang;Sun, Chengzhen;Yan, Liying;Liu, Bin;Zhang, Xuejun;Zhao, Zilong;Lai, Jinsheng;Xin, Ming

作者机构：

期刊名称：PLANT CELL （ 影响因子：10.0； 五年影响因子：11.1 ）

ISSN： 1040-4651

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite to fulfill breeding potential. Here, we found 2 SNPs in the E-class floral organ identity gene cucumber (Cucumis sativus) SEPALLATA2 (CsSEP2) that specifically regulate fruit length. Haplotype (HAP) 1 (8G2667A) and HAP2 (8G2667T) exist in natural populations, whereas HAP3 (8A2667T) is induced by ethyl methanesulfonate mutagenesis. Phenotypic characterization of 4 near-isogenic lines and a mutant line showed that HAP2 fruits are significantly longer than those of HAP1, and those of HAP3 are 37.8% longer than HAP2 fruit. The increasing fruit length in HAP1-3 was caused by a decreasing inhibitory effect on CRABS CLAW (CsCRC) transcription (a reported positive regulator of fruit length), resulting in enhanced cell expansion. Moreover, a 7638G/A-SNP in melon (Cucumis melo) CmSEP2 modulates fruit length in a natural melon population via the conserved SEP2-CRC module. Our findings provide a strategy for utilizing essential regulators with pleiotropic effects during crop breeding. Single-nucleotide changes in a flower development-related gene contribute to fruit length variations in cucurbits via differential inhibitory effects on transcription and cell expansion.

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