The Mh-miR393a-TIR1 module regulates Alternaria alternata resistance of Malus hupehensis mainly by modulating the auxin signaling
文献类型： 外文期刊
第一作者： Yu, Xinyi
作者： Yu, Xinyi;Hu, Kaixu;Geng, Xiaoyue;Cao, Lifang;Zhou, Tingting;Lin, Xinxin;Liu, Hongcheng;Chen, Jingrui;Qu, Shenchun;Geng, Xiaoyue;Luo, Changguo
作者机构：
关键词： MiR393a; TIR1; Malus hupehensis; Disease resistance; Apple; Alternaria alternata
期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）
ISSN： 0168-9452
年卷期： 2024 年 341 卷
页码：
收录情况： SCI
摘要： miRNAs govern gene expression and regulate plant defense. Alternaria alternata is a destructive fungal pathogen that damages apple. The wild apple germplasm Malus hupehensis is highly resistant to leaf spot disease caused by this fungus. Herein, we elucidated the regulatory and functional role of miR393a in apple resistance against A. alternata by targeting Transport Inhibitor Response 1. Mature miR393 accumulation in infected M. hupehensis increased owing to the transcriptional activation of MIR393a, determined to be a positive regulator of A. alternata resistance to either 'Orin' calli or 'Gala' leaves. 5 ' RLM-RACE and co -transformation assays showed that the target of miR393a was MhTIR1, a gene encoding a putative F -box auxin receptor that compromised apple immunity. RNA-seq analysis of transgenic calli revealed that MhTIR1 upregulated auxin signaling gene transcript levels and influenced phytohormone pathways and plant -pathogen interactions. miR393a compromised the sensitivity of several auxin -signaling genes to A. alternata infection, whereas MhTIR1 had the opposite effect. Using exogenous indole-3-acetic acid or the auxin synthesis inhibitor L-AOPP, we clarified that auxin enhances apple susceptibility to this pathogen. miR393a promotes SA biosynthesis and impedes pathogen -triggered ROS bursts by repressing TIR1-mediated auxin signaling. We uncovered the mechanism underlying the miR393a-TIR1 module, which interferes with apple defense against A. alternata by modulating the auxin signaling pathway.
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