Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation
文献类型： 外文期刊
第一作者： Liang, Xiaona
作者： Liang, Xiaona;Qiu, Yuliang;Wang, Ke;Xu, Zhiying;Yu, Mei;Du, Lipu;Lin, Zhishan;Ye, Xingguo;Bie, Xiaomin;Yang, Zujun;Jia, Yuqi;Lin, Zhishan;Ye, Xingguo
作者机构：
关键词： Genotype improvement; Plant regeneration ability; Transformation efficiency; Triticum aestivum
期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）
ISSN： 2095-5421
年卷期： 2023 年 11 卷 2 期
页码：
收录情况： SCI
摘要： Genetic transformation is widely used to improve target traits and to study gene function in wheat. However, transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew (PM) infection. The widely used recipient variety Fielder is very susceptible to PM. Therefore, it would be beneficial to develop PM resistant deriva-tives with high regeneration ability for use in genetic transformation. In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV, respectively, were backcrossed to Fielder with selection for PM resistance. Five lines, NT89, NT90, NT154, and WT48 with Pm21 and line FL347 with PmV were developed, identified by molecular markers and genomic in situ hybridization (GISH) or fluorescent in situ hybridization (FISH), and further subjected to detailed assessment of agronomic traits and regen-eration ability following genetic transformation capacity. Lines FL347, WT48, NT89 and NT154 assessed as being equal to, or superior, to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
分类号：
- 相关文献
作者其他论文 更多>>
-
Acidic calcium sulfate improved chilling tolerance and aroma quality by regulating reactive oxygen species metabolism and biosynthesis of volatiles in peaches
作者：He, Hui;Liu, Chen-xia;Wang, Qian-kun;Wang, Chun-fang;Chen, Bing-jie;Wang, Xiao;Qiao, Yong-jin;Liu, Hong-ru;Wang, Ke;Su, Jing-tang;Liu, Wen-hui;Li, Shao-zhen;Cheng, Zhao-hui
关键词：ACS; Chilling injury; Aroma volatiles; Antioxidant metabolism; Reactive oxygen species
-
Effects of crop rotation and straw return on soil microbiome in a southern paddy field
作者：Fang, Yu;Li, Qinghua;He, Chunmei;Wang, Ke;Wang, Fei
关键词：Brassica campestris; crop rotation; Glycine max; microbial community; Oryza sativa; paddy soil; straw return
-
Transcriptome analysis of muscle atrophy in Leizhou black goats: identification of key genes and insights into limb-girdle muscular dystrophy
作者：Wang, Ke;Han, Xiaotao;Liu, Hu;Han, Jiancheng;Zhou, Hanlin;Wang, Ke;Zhou, Hanlin;Xu, Mengning;Sun, Wei;Sun, Wei
关键词：Goat; Comparative transcriptome; Muscle atrophy
-
Missense Mutations in FDNC5 Associated with Morphometric Traits and Meat Quality in Hainan Black Goats
作者：Huang, Jing;Xu, Mengning;Han, Jiancheng;Zhou, Hanlin;Wang, Ke;Huang, Jing;Xu, Mengning;Zhang, Yuelang;Zhou, Hanlin;Wang, Ke
关键词：
FNDC5 ; missense mutation; morphometric traits; meat quality; goat -
Auxin-Producing Pseudomonas Recruited by Root Flavonoids Increases Rice Rhizosheath Formation through the Bacterial Histidine Kinase Under Soil Drying
作者：Xu, Feiyun;Wang, Yongsen;Yang, Jinyong;Zhang, Xue;Tong, Lu;Bai, Chuqi;Chen, Shu;Sun, Leyun;Du, Chongxuan;Fang, Ju;Gengli, Jiahong;Liu, Jianping;Xu, Weifeng;Zhang, Xue;Wang, Ke;Ding, Fan;Xu, Mengqiang;Li, Liang;Zhang, Qian;Wang, Zhengrui;Pang, Jiayin;Yu, Xin;Zhu, Yiyong;Zhang-Zheng, Huanyuan;Zhang-Zheng, Huanyuan;Zhang, Jianhua
关键词：polyploidy; pseudomonas; rhizosheath formation; rice; soil drying
-
Identification and characterization of a broadly neutralizing and protective nanobody against the HA1 domain of H5 avian influenza virus hemagglutinin
作者：Xu, Siqi;Liu, Yutong;Zhou, Mengruo;Wang, Ke;Xie, Qianmei;Zhang, Qi;Zhang, Qinying;Li, Qianyu;Pan, Zhichao;Feng, Saixiang;Liao, Ming;Luo, Chenying;Liao, Ming;Liao, Ming
关键词：influenza virus; yeast two-hybrid; nanobody; broadly neutralizing; surface plasmon resonance; inhibition mechanism; prophylactic; therapeutic; epitope mapping
-
Near-freezing temperature alters ROS metabolism and gene transcription to enhance peach chilling tolerance and aroma quality
作者：Liu, Hongru;He, Hui;Wang, Qiankun;Liu, Chenxia;Wang, Chunfang;Wang, Xiao;Chen, Bingjie;Zhang, Yi;Qiao, Yongjin;Li, Shaozhen;Guo, Fengjun;Zhang, Changfeng;Guo, Fengjun;Zhang, Changfeng;Wang, Ke;Hu, Liushen;Liu, Hongru
关键词：Peach; NFT; Chilling injury; Aroma volatiles; ROS metabolism; Gene expression
