Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation

文献类型： 外文期刊

第一作者： Liang, Xiaona

作者： Liang, Xiaona;Qiu, Yuliang;Wang, Ke;Xu, Zhiying;Yu, Mei;Du, Lipu;Lin, Zhishan;Ye, Xingguo;Bie, Xiaomin;Yang, Zujun;Jia, Yuqi;Lin, Zhishan;Ye, Xingguo

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关键词： Genotype improvement; Plant regeneration ability; Transformation efficiency; Triticum aestivum

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Genetic transformation is widely used to improve target traits and to study gene function in wheat. However, transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew (PM) infection. The widely used recipient variety Fielder is very susceptible to PM. Therefore, it would be beneficial to develop PM resistant deriva-tives with high regeneration ability for use in genetic transformation. In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV, respectively, were backcrossed to Fielder with selection for PM resistance. Five lines, NT89, NT90, NT154, and WT48 with Pm21 and line FL347 with PmV were developed, identified by molecular markers and genomic in situ hybridization (GISH) or fluorescent in situ hybridization (FISH), and further subjected to detailed assessment of agronomic traits and regen-eration ability following genetic transformation capacity. Lines FL347, WT48, NT89 and NT154 assessed as being equal to, or superior, to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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