Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database

文献类型： 外文期刊

第一作者： Niu, BL

作者： Niu, BL;Meng, ZQ;Tao, YZ;Lu, SL;Weng, HB;He, LH;Shen, WF

作者机构：

关键词： EST database;transformer-2 gene;Bmtra-2;alternative splicing;gene clone;Bombyx mori

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

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收录情况： SCI

摘要： We have identified Bombyx mori transformer-2 gene(Bmtra-2)cDNA by blasting the EST database of B.mori.It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis.By comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns,and all splice sites of exons/introns conformed to the GT/AG rule.Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing.Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction.All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif,showing striking organizational similarity to Drosophila TRA-2 proteins.

分类号： Q7

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