Antigenic determination fragments of alpha-momorcharin

文献类型： 外文期刊

第一作者： Leung, KC

作者： Leung, KC;Meng, ZQ;Ho, WKK

作者机构：

关键词： Ribosome-inactivating protein;Antigenicity;Protein structure;Random fragment;Alpha-momorcharin;Trichosanthin

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS （ 影响因子：3.77； 五年影响因子：4.137 ）

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年卷期：

页码：

收录情况： SCI

摘要： Alpha momorcharin is a protein isolated from the bitter gourd. It has a number of biological activities including induction of abortion, inhibition of tumor growth and anti-HIV. All these activities may be related to the ribosome-inhibiting activity of the protein. Repeated use of αMMC can elicit an antigenic response which may neutralize its biological activity. To overcome this problem, we need to know which part of the molecule is the antigenic determinant. In this study, we constructed a random fragment expression library from the αMMC cDNA and screened it with three anti-αMMC sera. A total of 9 positive clones were picked and sequenced. Based on the sequence information obtained, we were able to deduce three regions at which antibodies raised against native αMMC seem to interact. These regions are residues 1–14, residues 71–136 and residues 195–222. Mapping of these regions against a 3D model of αMMC indicates that they all are located on the surface of the molecule. As residues 71–136 are found to be in close proximity to the active site involved in ribosome inactivation, treatment with a monoclonal antibody directed to this area was shown to be effective in inactivating the inhibitory effect of αMMC on in vitro protein synthesis.

分类号： Q5

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