Virus-Induced Gene Silencing (VIGS) in Hydrangea macrophylla and Functional Analysis of HmF3′5′H
文献类型： 外文期刊
第一作者： Yang, Qiyu
作者： Yang, Qiyu;Fan, Youwei;Luo, Shuwen;Liu, Chun;Yuan, Suxia
作者机构：
关键词：
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 23 期
页码：
收录情况： SCI
摘要： Hydrangea macrophylla, renowned for its large inflorescences and a diverse range of colors, highlights a significant limitation in current gene function research, which is the lack of effective molecular genetic tools. This study utilized a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate gene function through posttranscriptional gene silencing in H. macrophylla for the first time. The ortholog of phytoene desaturase (PDS) in H. macrophylla, termed HmPDS, was identified. Infection of tissue-cultured seedlings with TRV-HmPDS led to photobleaching of the leaves. Additionally, infection with TRV containing the HmCHS1 fragment in the flowers resulted in decreased anthocyanin production in sepals and a lightening of sepal coloration in the infected flowers. The phenomena and RT-qPCR results proved that the PDS and CHS genes of hydrangea were successfully silenced via the vacuum infiltration method. Furthermore, the introduction of TRV-HmF3 ' 5 ' H revealed a decrease in delphinidin-3-glucoside content in sepals and caused a color change in the sepals from blue to pink. This study demonstrated that the TRV-VIGS system was successfully established in H. macrophylla and effectively applied to the function analysis of HmF3 ' 5 ' H.
分类号：
- 相关文献
作者其他论文 更多>>
-
Enhanced virulence of Acinetobacter johnsonii at low temperatures induces acute immune response and systemic infection in American bullfrogs (Aquarana catesbeiana)
作者：Lin, Han;Sun, Jingyang;Li, Hua;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Cold stress; Virulence genes; Amphibian disease; Immune response; Systemic infection
-
Enhancing cooking and eating quality of semi-dried brown rice noodles through Lactobacillus fermentation and moderate lysine addition
作者：Luo, Lijuan;Xiong, Gangping;Wang, Qing;Xiang, Xiongzi;Long, Zhao;Huang, Zhengyu;Ding, Yuqin;Liu, Chun;Luo, Lijuan
关键词：Fermented Brown Rice noodles; Lysine addition; Sensory evaluation; Cooking quality; Texture
-
Frog virus 3: Prevalence survey and quantitative analysis in American bullfrogs using TaqMan MGB probe real-time fluorescence quantitative PCR
作者：Li, Hua;Liu, Xiaobing;Sun, Jingyang;Lin, Han;Chen, Kesong;Hu, Yueming;Jiang, Mengzhen;Jiang, Biao;Li, Wei;Su, Youlu;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Aquarana catesbeiana; Frog virus 3; TaqMan-MGB real-time PCR; Virus load; Prevalence survey
-
Genome-Wide Identification of Expansin Gene Family and Its Association with Sepal Expansion During Flower Opening in Hydrangea macrophylla
作者：Fan, Youwei;Yue, Dan;Liang, Zhongshuo;Liu, Chun;Yuan, Suxia;Fan, Youwei;Liu, Chun;Yuan, Suxia;Yue, Dan
关键词：Hydrangea macrophylla; Expansins; Gene family; Sepal development; Gene expression profiles
-
Archidendron clypearia extract: Modulating intestinal inflammation and enhancing disease resistance in aquacultured American bullfrogs (Aquarana catesbeiana)
作者：Chen, Kesong;Lin, Han;Sun, Jingyang;Li, Hua;Ma, Jie;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing
关键词：American bullfrogs; Archidendron clypearia; Disease resistance; Intestinal inflammation
-
A chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation
作者：Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Luo, Lijuan;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Luo, Lijuan;Liu, Pandao;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Luo, Lijuan;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Luo, Lijuan;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Liu, Guodao;Liu, Pandao;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Liu, Pandao;Liu, Chun;Zhang, Jianyu;Xu, Ranran;Lv, Jinhui;Liu, Pandao;Qiao, Zhu;Bai, Mingzhou;Zhao, Shancen
关键词：Stylosanthes angustifolia; pioneer plant; de novo assembly; multiomics; drought tolerance
-
Comparative analysis of lipid and flavonoid biosynthesis between Pongamia and soybean seeds: genomic, transcriptional, and metabolic perspectives
作者：Liu, Chun;Huang, Rui;Li, Xinyong;Liu, Guodao;Dong, Rongshu;Liu, Pandao;Liu, Chun;Huang, Rui;Li, Xinyong;Liu, Guodao;Dong, Rongshu;Liu, Pandao;Liu, Chun;Liu, Pandao;Liu, Chun;Liu, Pandao;Liu, Chun;Zhao, Xingkun;Xu, Ranran;Zhang, Jianyu;Liu, Chun;Zhao, Xingkun;Xu, Ranran;Zhang, Jianyu
关键词：Pongamia; Soybean; Multi-omics; Lipids; Flavonoids
