Cyprinid herpesvirus 2 ORF41 Protein Degrades Pyruvate Dehydrogenase (PDH)-E1β to Promote Viral Replication in Gibel Carp Brain (GiCB) Cells

文献类型： 外文期刊

第一作者： Xue, Mingyang

作者： Xue, Mingyang;Xu, Chen;Huang, Zhenyu;Meng, Yan;Jiang, Nan;Fan, Yuding;Zhou, Yong;Xue, Mingyang

作者机构：

关键词： Cyprinid herpesvirus 2; ORF41; pyruvate dehydrogenase

期刊名称：FISHES （ 影响因子：2.4； 五年影响因子：2.4 ）

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年卷期： 2025 年 10 卷 3 期

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收录情况： SCI

摘要： Cyprinid herpesvirus 2 (CyHV-2) is a major pathogen posing a serious threat to crucian carp farming and has led to major economic losses in China's aquaculture industry. This research aimed to explore how the CyHV-2-ORF41 protein influences viral replication. Firstly, we found that ORF41 overexpression in Gibel carp brain (GiCB) cells significantly enhanced CyHV-2 replication. Subsequently, GST pull-down and LC-MS/MS analyses were conducted to identify ORF41's protein interactions. The results showed that ORF41 might interact with pyruvate dehydrogenase (PDH)-E1 beta, an enzyme connecting glycolysis to the tricarboxylic acid (TCA) cycle. Furthermore, ORF41 expression decreased the PDH-E1 beta levels, leading to pyruvate and lactic acid accumulation. Molecular docking and dynamics simulations confirmed a stable interaction between ORF41 and PDH-E1 beta. This research not only deepens our understanding of CyHV-2's mechanisms of infection but also suggests potential targets for therapeutic strategies in aquaculture.

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