Fine Mapping and Characterization of a Major Gene Responsible for Chlorophyll Biosynthesis in Brassica napus L.

文献类型： 外文期刊

第一作者： Pang, Chengke

作者： Pang, Chengke;Peng, Menlu;Zhao, Xiaozhen;Shi, Rui;Zhang, Jiefu;Pang, Chengke;Zhang, Wei;Peng, Menlu;Zhao, Xiaozhen;Shi, Rui;Wu, Xu;Chen, Feng;Sun, Chengming;Wang, Xiaodong;Wu, Xu;Zhang, Jiefu

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关键词： Brassica napus; quantitative trait loci sequencing; candidate gene; chlorophyll deficiency; fine mapping; molecular marker

期刊名称：BIOMOLECULES （ 影响因子：6.064； 五年影响因子：6.191 ）

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年卷期： 2022 年 12 卷 3 期

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收录情况： SCI

摘要： Rapeseed (Brassica napus L.) is mainly used for oil production and industrial purposes. A high photosynthetic efficiency is the premise of a high yield capable of meeting people's various demands. Chlorophyll-deficient mutants are ideal materials for studying chlorophyll biosynthesis and photosynthesis. In a previous study, we obtained the mutant yl1 for leaf yellowing throughout the growth period by ethyl methanesulfonate mutagenesis of B. napus. A genetic analysis showed that the yl1 chlorophyll-deficient phenotype was controlled by one incompletely dominant gene, which was mapped on chromosome A03 by a quantitative trait loci sequencing analysis and designated as BnA03.Chd in this study. We constructed an F-2 population containing 5256 individuals to clone BnA03.Chd. Finally, BnA03.Chd was fine-mapped to a 304.7 kb interval of the B. napus 'ZS11' genome containing 58 annotated genes. Functional annotation, transcriptome, and sequence variation analyses confirmed that BnaA03g0054400ZS, a homolog of AT5G13630, was the most likely candidate gene. BnaA03g0054400ZS encodes the H subunit of Mg-chelatase. A sequence analysis revealed a single-nucleotide polymorphism (SNP), causing an amino-acid substitution from glutamic acid to lysine (Glu1349Lys). In addition, the molecular marker BnaYL1 was developed based on the SNP of BnA03.Chd, which perfectly cosegregated with the chlorophyll-deficient phenotype in two different F-2 populations. Our results provide insight into the molecular mechanism underlying chlorophyll synthesis in B. napus.

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