RNAi assay in the 28-spotted ladybeetle Henosepilachna vigintioctopunctata suggests nuclear receptor HR3 as a potential molecular target for pest control

文献类型： 外文期刊

第一作者： Li, Zhaoyang

作者： Li, Zhaoyang;Ruan, Yalin;Zhou, Chuqiao;Liu, Junna;Wang, Yajie;Pan, Huipeng;Yoon, June-Sun;Zhou, Xuguo;Zhang, Youjun;Yang, Chunxiao

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关键词： RNA interference; pest control; hormone receptor 3; 28-spotted ladybeetle; developmental stage

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2025 年 81 卷 8 期

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收录情况： SCI

摘要： BACKGROUND Nuclear hormone receptors play crucial roles in embryonic development, neural regulation, metabolism, and organogenesis in insects. This study aimed to investigate the expression and function of the nuclear hormone receptor 3 (HR3) in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata. RESULTS Our findings revealed that HvHR3 was expressed at all developmental stages, with the highest expression levels in the 1st instar larvae, 3rd instar larvae, and pupa. HvHR3 silencing in both the 1st and 3rd instar larvae resulted in high mortality rates. Reduced HvHR3 expression in the 3rd instar larvae led to developmental arrest and failure to molt, accompanied by a down-regulation of 20E-related genes. Furthermore, HvHR3 silencing in the 3rd instar larvae resulted in a decrease in the 20E titer. Silencing HvHR3 in adult females significantly impaired ovarian development, resulting in reduced ovary size and absence of mature oocytes. Additionally, the eggs produced by these females had partially ruptured shells and failed to maintain normal spindle shape. CONCLUSION These findings indicate that HvHR3 is a critical regulator of development, molting, and reproduction, underscoring its potential as a molecular target for RNA interference-based biopesticides aimed at controlling this pest. (c) 2025 Society of Chemical Industry.

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