The vacuole pH-related gene RcNHX2 affects flower color shift and Na plus homeostasis in roses

文献类型： 外文期刊

第一作者： Wang, Bingshuang

作者： Wang, Bingshuang;Wang, Huanhuan;Liu, Minghui;He, Guoren;Ming, Feng;Wang, Bingshuang;Wang, Huanhuan;Liu, Minghui;He, Guoren;Ming, Feng

作者机构：

关键词： Rose; Na plus /H plus transporter; Flower color; Na plus homeostasis

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2025 年 357 卷

页码：

收录情况： SCI

摘要： Rose (Rosa spp.) is one of the most famous ornamental plants in the world, and its commodity value largely depends on its flower color. The color of roses mainly depends on the composition and state of anthocyanins, and the vacuolar pH value is an important factor affecting the stability and state of anthocyanins. The vacuolar sodium/proton antiporters (NHXs) play important roles in the maintenance of cellular ion homeostasis and petal vacuolar pH. However, the NHX functions related to rose flower coloration remain relatively uncharacterized. In this study, we cloned and characterized the vacuolar pH-related gene RcNHX2, which encoded a vesicular cation/H+ antiporter protein. Phylogenetic sequence analysis revealed that RcNHX2 belongs to the vesicular NHX family of proteins. It is localized in the vesicular membrane, where it exerts its function. RcNHX2 was significantly differentially expressed in different color-presenting types of petals of roses, and it was particularly highly expressed in the blue-purple petals. The overexpression of RcNHX2 in Rosa hybrida 'Florentina' caused the pH to increase and the petal color to change from red to blue-purple. On the basis of virus-induced gene silencing, we determined that decreased RcNHX2 expression significantly reduces R. hybrida 'Blue For You' petal coloration. We indicated that RcNHX2 might be involved in the color shift to blue in roses. Moreover, it was observed that in the cells of the rose plants in which RcNHX2 was silenced, the Na+ homeostasis was affected. The results suggest that the vesicular Na+/H+ transporter, RcNHX2 gene, likely plays a crucial role in the blue color change and the maintenance of cellular Na+ homeostasis in roses. These findings offer valuable insights for the cultivation of blue rose.

分类号：

  • 相关文献
作者其他论文 更多>>
  • PeTB1/2 and PeSLR1 mediate the mechanism of PePIN1b involved in axillary bud dormancy in Phalaenopsis

    作者：Meng, Yang;He, Guoren;Zhang, Qiyu;Wang, Mengyi;Lou, Yuxia;Ming, Feng;Meng, Yang;He, Guoren;Zhang, Qiyu;Wang, Mengyi;Lou, Yuxia;Ming, Feng

    关键词：Phalaenopsis; Axillary bud dormancy; Phytohormone; CYC/TB1; DELLA; PePIN1b

  • SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress

    作者：Huang, Ruihua;Wen, Mengrui;Feng, Bojin;Zhong, Xiaoqing;Yang, Yifeng;Liu, Minghui;Li, Hongqing;Yang, Chengwei;Peng, Changlian;Zhang, Shengchun;Wu, Pingzhi

    关键词：

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Field-Based Evaluation of Insecticide Effectiveness on Megalurothrips usitatus in Guangdong, China: Implications for Pest Control Strategies

    作者：Peng, Zhengke;Li, Mengni;Guo, Chaosong;Yin, Fei;Xiao, Yong;Wang, Huanhuan;Li, Zhenyu;Guo, Chaosong;Zheng, Huixin;Wu, Mingyue;Kong, Xiangyi;Zalucki, Myron P.;Xie, Wen;Xie, Wen

    关键词：cowpea; bean flower thrips; insecticide toxicity monitoring; resistance management; IPM

  • Whole-genome variants dataset of 209 local chickens from China

    作者：Tan, Xiaodong;Zhang, Jiawen;Dong, Jie;Huang, Minjie;Zhou, Zhenzhen;Yang, Shuyuan;Wang, Deqian;Li, Qinghai;Wang, Huanhuan;Bai, Lijuan;Cui, Ming

    关键词：

  • Two cuticle-enriched chemosensory proteins confer multi-insecticide resistance in Spodoptera frugiperda

    作者：Wang, Huanhuan;Zhao, Rui;Gao, Jie;Xiao, Xing;Yin, Xinhui;Hu, Shiyuan;Liang, Pei;Gu, Shaohua;Zhang, Yongjun

    关键词：Spodoptera frugiperda; CSPs; Insecticide binding; Lepidoptera pest; Penetration resistance; Insecticide sequestration mechanism

  • PeFtsH5 negatively regulates the biological stress response in Phalaenopsis equestris mitochondria

    作者：Wu, Yiding;Meng, Yang;Zhang, Long;Yang, Zhenyu;Su, Mingyang;Ming, Feng;Wu, Yiding;Meng, Yang;Zhang, Long;Yang, Zhenyu;Su, Mingyang;Ming, Feng;Jia, Ruidong;Zhang, Long

    关键词：Phalaenopsis equestris; Mitochondria; FtsH5; Virus induced gene silencing (VIGS); Cell apoptosis

微信小程序