SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress

文献类型： 外文期刊

第一作者： Huang, Ruihua

作者： Huang, Ruihua;Wen, Mengrui;Feng, Bojin;Zhong, Xiaoqing;Yang, Yifeng;Liu, Minghui;Li, Hongqing;Yang, Chengwei;Peng, Changlian;Zhang, Shengchun;Wu, Pingzhi

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.1 ）

ISSN： 1040-4651

年卷期： 2025 年 37 卷 5 期

页码：

收录情况： SCI

摘要： High-temperature stress hinders seed filling, reducing seed quality and crop yield. However, the molecular mechanisms underlying this process remain unclear. Here, we identify SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) as a key regulator of seed filling under prolonged high temperatures in Arabidopsis (Arabidopsis thaliana). SIZ1 and WRINKLED1 (WRI1) are co-expressed during seed filling, and overexpressing either gene enhances seed filling and promotes fatty acid biosynthesis under high-temperature stress. Genetic and biochemical analyses revealed that SIZ1 stabilizes WRI1 by promoting its SUMOylation at Lys-257 and Lys-266, thereby inhibiting its interaction with the CULLIN3-based ubiquitin E3 ligase adaptor protein BTB/POZMATH (BPM) and preventing its ubiquitination and degradation. Mutating these SUMOylation sites accelerates WRI1 degradation, impairing its function in seed filling under high-temperature stress. Furthermore, high-temperature stress induces SIZ1 expression and reduces WRI1 levels, suggesting that SIZ1-mediated SUMOylation counteracts high-temperature stress-induced WRI1 instability. These findings establish SIZ1 as a crucial factor in maintaining WRI1 stability and seed filling under high-temperature stress, providing valuable genetic resources and a theoretical foundation for addressing prolonged high-temperature stress in agricultural production. SUMOylation by the E3 SUMO ligase SIZ1 prevents the ubiquitination of the transcription factor WRINKLED1 and ensures its stability, thereby safeguarding seed filling and fatty acid biosynthesis at high temperatures.

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