Screening and characterization of sex-linked DNA markers in Mozambique tilapia (Oreochromis mossambicus)

文献类型： 外文期刊

第一作者： Tao, Wenjing

作者： Tao, Wenjing;Zhu, Xi;Xiao, Hesheng;Wang, Deshou;Cao, Jianmeng;Dong, Junjian;Lu, Maixin;Kocher, Thomas D.

作者机构：

关键词： Mozambique tilapia; Genome sequencing and re-sequencing; Sex-linked markers; GMT (genetically male tilapia); Cichlid fish

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 557 卷

页码：

收录情况： SCI

摘要： The Mozambique tilapia (Oreochromis mossambicus) is an important aquaculture fish. It shows sexual dimorphism in growth, with males growing much faster than females. Thus, production of all-male fingerlings of Mozambique tilapia would improve aquaculture efficiency. However, the lack of accurate sex-specific markers has hampered the mono-sex breeding and the study of the molecular mechanisms of sex determination in this species. Recently, we performed genome sequencing and re-sequencing of female and male Mozambique tilapia and identified the sex chromosome and sex determining region. In the present study, two sex-linked SNP markers were identified and validated. Male specific contigs were assembled from the unmapped reads after aligning male re-sequencing data against the published XX genome. Based on these male-specific contigs, three sex-linked DNA markers, Marker-1, Marker-2 and Marker-3, were developed and correctly identified the genetic sex of 16 fish. The universality of these three male-specific markers was then tested in a panel of 144 individuals, which showed a discrepancy of 5.5% (8/144) between genotypic sex and phenotypic sex. In conclusion, we developed three sexlinked markers to identify different sex genotypes in Mozambique tilapia. This study lays a foundation for the establishment of MAS-GMT (marker-assisted selection of genetically male tilapias) technique and sex-controlled breeding of Mozambique tilapia, as well as for the identification of the sex determining gene.

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