Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit

文献类型： 外文期刊

第一作者： Chen, Jing

作者： Chen, Jing;Tan, Jing;Duan, Xinyu;Wang, Ying;Wen, Jing;Li, Zhengguo;Xu, Haiyang;Li, Wei;Li, Wei;Wang, Guodong;Wang, Guodong

作者机构：

关键词： Plastid engineering; Farnesyl diphosphate; Fruit-specific metabolic engineering; MEP pathway; Sesquiterpene; Patchouli alcohol

期刊名称：METABOLIC ENGINEERING （ 影响因子：8.4； 五年影响因子：8.7 ）

ISSN： 1096-7176

年卷期： 2023 年 77 卷

页码：

收录情况： SCI

摘要： Sesquiterpenes represent a large class of terpene compounds found in plants with broad applications such as pharmaceuticals and biofuels. The plastidial MEP pathway in ripening tomato fruit is naturally optimized to provide the 5-carbon isoprene building blocks of all terpenes for production of the tetraterpene pigment lycopene and other carotenoids, making it an excellent plant system to be engineered for production of high-value ter-penoids. We reconstituted and enhanced the pool of sesquiterpene precursor farnesyl diphosphate (FPP) in plastids of tomato fruit by overexpressing the fusion gene DXS-FPPS encoding a fusion protein of 1-deoxy-D-xylu-lose 5-phosphate synthase (DXS) linked with farnesyl diphosphate synthase (originally called farnesyl pyro-phosphate synthase, and abbreviated as FPPS) under the control of fruit-ripening specific polygalacturonase (PG) promoter concomitant with substantial reduction in lycopene content and large production of FPP-derived squalene. The supply of precursors achieved by the fusion gene expression can be harnessed by an engineered sesquiterpene synthase that is retargeted to plastid to engineer high-yield sesquiterpene production in tomato fruit, offering an effective production system for high-value sesquiterpene ingredients.

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