Roles of MEF2A and HOXA5 in the transcriptional regulation of the bovine FoxO1 gene

文献类型： 外文期刊

第一作者： Wei, Dawei

作者： Wei, Dawei;Yaping, Song;Chao, Jiang;Wang, Jin;Zhang, Jiupan;Khan, Rajwali;Abbas Raza, Sayed Haidar;Ayari-Akkari, Amel;Ayari-Akkari, Amel;Ahmed, Dalia Abd El Moneim;Khan, Rajwali

作者机构：

关键词： FoxO1 gene; transcription factors; MEF2A; HOXA5; cloning

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）

ISSN： 1049-5398

年卷期： 2022 年

页码：

收录情况： SCI

摘要： The Forkhead box factor 1 (FoxO1) gene plays a vital role in the growth and development of skeletal muscle. In the present study, expression analysis of the bovine FoxO1 gene exhibited the highest expression in longissimus dorsi muscle followed by its expression in adipose tissue. Moreover, high mRNA expression of FoxO1 gene was found in differentiated bovine myoblasts and adipocytes at day 6 of induced differentiation (p < 0.05). The regulatory pattern of the bovine FoxO1 gene was investigated through screening and dual-luciferase activity of the 1.7 kb 5 ' UTR (untranslated region) within pGL3-basic vector and a core promoter region was explored at (-285/-27) upstream of the transcription start site. The transcription factors (TFs) MEF2A and HOXA5 within the core promoter region (-285/-27) were found as the regulatory cis-acting element. The siRNA interference of the TFs, chromatin immunoprecipitation (ChIP) assay, and site-directed mutation validated that MEF2A and HOXA5 binding occurs in the region -285/-27 bp and performs an essential role in the transcriptional regulation of bovine FoxO1 gene. These findings explored the regulatory network mechanism of the FoxO1 gene in skeletal muscle development and adipogenesis for the bovine breed improvement program.

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