Synergistic Effects and Mechanisms of Action of Rutin with Conventional Antibiotics Against Escherichia coli
文献类型： 外文期刊
第一作者： Yi, Lankun
作者： Yi, Lankun;Bai, Yubin;Chen, Xu;Wang, Weiwei;Zhang, Chao;Shang, Zixuan;Zhang, Zhijin;Li, Jiajing;Zhang, Jiyu;Yi, Lankun;Chen, Xu;Shang, Zixuan;Zhang, Zhijin;Li, Jiajing;Cao, Mingze;Zhu, Zhen
作者机构：
关键词：
rutin; amikacin;
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 24 期
页码：
收录情况： SCI
摘要： Rutin is a widely known plant secondary metabolite that exhibits multiple physiological functions. The present study focused on screening for synergistic antibacterial combinations containing rutin, and further explored the mechanisms behind this synergy. In vitro antibacterial test results of rutin showed that the ranges of minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) are 0.125-1 and 0.125-2 mg/mL, respectively. However, rutin and amikacin have a significant synergistic effect, with a fractional inhibitory concentration index (FICI) range of 0.1875-0.5. The time bactericidal curve proved that the combination of rutin and amikacin inhibited bacterial growth within 8 h. Scanning electron microscopy (SEM) revealed that a low-dose combination treatment could disrupt the cell membrane of Escherichia coli (E. coli). A comprehensive analysis using alkaline phosphatase (AKP), K+, and a protein leakage assay revealed that co-treatment destroyed the cell membrane of E. coli, resulting in the significant leakage of AKP, intracellular K+, and proteins. Moreover, confocal laser scanning microscopy (CLSM) and red-green cell ratio analysis indicated severe damage to the E. coli cell membrane following the co-treatment of rutin and amikacin. This study indicates the remarkable potential of strategically selecting antibacterial agents with maximum synergistic effect, which could significantly control antibiotic resistance.
分类号：
- 相关文献
作者其他论文 更多>>
-
Design, synthesis, and antibacterial activity of novel amide derivatives containing a sulfone moiety
作者：Zou, Yue;Liu, Xing;Zhu, Zongnan;Zhang, Yong;Chen, Jixiang;Zhang, Chao;Zhao, Yuanzheng;Zhu, Xiang
关键词：Sulfone; Amide; Antibacterial activity; Structure-activity relationship
-
D-mannose-modified nanoliposomes enhance the targeted delivery of ovalbumin to improve its anti-inflammatory, antioxidant, and macrophage polarization effects
作者：Liao, Jiahao;Chi, Xiaoyi;Wang, Weiwei;Huang, Jianyu;Li, Xiaoqiong;Kuang, Jian;Li, Jianqiang;Bian, Xiangyu;Li, Jinjun;Pang, Jinzhu;Wang, Weiwei
关键词：D-mannose nanoliposome; Ovalbumin delivery; Macrophage targeting; Anti-inflammatory effects
-
Pleiotropic effects of Ebony on pigmentation and development in the Asian multi-coloured ladybird beetle, Harmonia axyridis (Coleoptera: Coccinellidae)
作者：Lin, Jing;Wu, Mengmeng;Chen, Xu;Zang, Liansheng;Lin, Jing;Wu, Mengmeng;Chen, Xu;Zang, Liansheng;Lin, Jing;Xiao, Da;Xu, Qingxuan;Wang, Su;Xiao, Da;Xu, Qingxuan;Wang, Su
关键词：CRISPR/Cas9;
Ebony ;Harmonia axyridis ; pigmentation -
Effect of temperature regulation on microbial community, volatile flavours, amino acid profiles, and iridoid glycosides during noni (Morinda citrifolia L.) fruit fermentation
作者：Zhang, Chao;Gu, Chunhe;Wang, Mengrui;Chen, Junxia;Chang, Haode;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Zhang, Wenjing;Zhang, Quanmiao;Feng, Zhen;Zhang, Chao;Wang, Mengrui;Chen, Junxia;Chang, Haode;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Zhang, Wenjing;Zhang, Quanmiao;Feng, Zhen;Gu, Chunhe;Feng, Zhen
关键词：Shotgun metagenomics; Noni fruit; Volatile flavours; Amino acid profiles; Iridoid glycosides
-
Effect of hot-air drying processing on the volatile organic compounds and maillard precursors of Dictyophora Rubrovalvata based on GC-IMS, HPLC and LC-MS
作者：Meng, Lingshuai;Nie, Yu;Zheng, Tingting;Chen, Haijiang;Lin, Dong;Cao, Sen;Xu, Su;Zhou, Qingsong;Song, Jianxin;Zhang, Chao;Meng, Lingshuai;Nie, Yu;Zheng, Tingting;Chen, Haijiang;Lin, Dong;Xu, Su
关键词：Dictyophora rubrovalvata; Hot air drying; Maillard reaction; Precursor substances; Key aroma substances; GC-IMS
-
Bioremediation of complex organic pollutants by engineered Vibrio natriegens
作者：Su, Cong;Liu, Yong;Wang, Chen;Qu, Liwen;Dai, Junbiao;Cui, Haotian;Wang, Weiwei;Cheng, Zhenyu;Yang, Mengqiao;Li, Ye;He, Siyang;Xu, Ping;Tang, Hongzhi;Cui, Haotian;Wang, Weiwei;Cheng, Zhenyu;Yang, Mengqiao;Li, Ye;He, Siyang;Xu, Ping;Tang, Hongzhi;Cai, Yuejin;Zheng, Jiaxin;Zhao, Pingping;Dai, Junbiao
关键词：
-
Moderate Nitrogen Application Synergistically Improved Yield and Quality of Nanjing Series japonica Rice Varieties with Good Taste
作者：Wei, Xiaodong;Zhao, Qingyong;Zhao, Chunfang;Zhang, Yong;Chen, Tao;Zhu, Zhen;Lu, Kai;He, Lei;Zhou, Lihui;Huang, Shengdong;Li, Yusheng;Wang, Cailin;Zhang, Yadong;Wang, Cailin;Zhang, Yadong
关键词：nitrogen level; japonica rice; high-quality cultivation; grain yield; good eating quality
