Single-nucleus transcriptomics reveal cardiac cell type-specific diversification in metabolic disease transgenic pigs

文献类型： 外文期刊

第一作者： Miao, Jiakun

作者： Miao, Jiakun;Zhang, Kaiyi;Yang, Yu;Xu, Shuang;Du, Juan;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Yang, Shulin

作者机构：

期刊名称：ISCIENCE （ 影响因子：4.6； 五年影响因子：5.0 ）

ISSN：

年卷期： 2024 年 27 卷 6 期

页码：

收录情况： SCI

摘要： Cardiac damage is widely present in patients with metabolic diseases, but the exact pathophysiological mechanisms involved remain unclear. The porcine heart is an ideal material for cardiovascular research due to its similarities to the human heart. This study evaluated pathological features and performed single -nucleus RNA sequencing (snRNA-seq) on myocardial samples from both wild -type and metabolic disease -susceptible transgenic pigs (previously established). We found that transgenic pigs exhibited lipid metabolism disturbances and myocardial injury after a high -fat high -sucrose diet intervention. snRNAseq reveals the cellular landscape of healthy and metabolically disturbed pig hearts and identifies the major cardiac cell populations affected by metabolic diseases. Within metabolic disorder hearts, metabolically active cardiomyocytes exhibited impaired function and reduced abundance. Moreover, massive numbers of reparative LYVE1 + macrophages were lost. Additionally, proinflammatory endothelial cells were activated with high expression of multiple proinflammatory cytokines. Our findings provide insights into the cellular mechanisms of metabolic disease -induced myocardial injury.

分类号：

  • 相关文献
作者其他论文 更多>>
  • A rapid and highly sensitive CRISPR assay utilizing Cas12a orthologs for the detection of Novel Duck Reovirus

    作者：Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Cui;He, Daqian;Gong, Ping;Yang, Yu

    关键词：NDRV; Cas12a orthologs; Gs12-16; Gs12-18; CRISPR-based diagnostics

  • Ethanol inhibited growth hormone receptor-mediated endocytosis in primary mouse hepatocytes

    作者：Ge, Zihan;Liu, Xingjie;Zhang, Wei;Xian, Li;Li, Ruonan;Ge, Zihan;Yang, Yu;Lan, Hainan;Zhang, Yawen

    关键词：growth hormone; growth hormone receptor; ethanol; nuclear translocation; endocytosis

  • Molecular pathological characteristics and mechanisms of the liver in metabolic disease-susceptible transgenic pigs

    作者：Du, Juan;Zhang, Kaiyi;Miao, Jiakun;Yang, Yu;Tian, Yuying;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Yang, Shulin

    关键词：Transgenic pigs; ETNPPL; GABBR2; BMP8B; Bis (monoacylglycerol) phosphates; Sulfatides

  • Cadmium exposure induced spleen inflammation by activating the MAPK/ NF-κB/ NLRP3 signaling pathway and the intervention effect of astilbin

    作者：Chen, Tiezhu;Yan, Liangchun;Dong, Han;Cheng, Tingting;Cheng, Guoqiang;Han, Tianyu;Liu, Zhijun;Liu, Yiding;Yang, Yu;Fei, Shanshan;Shi, Guangliang;Miao, Yusong;Chen, Tiezhu;Han, Tianyu;Liu, Zhijun;Liu, Yiding;Yang, Yu;Fei, Shanshan;Shi, Guangliang;Yan, Liangchun

    关键词：Cadmium; Astilbin; Oxidative stress; Inflammation; Spleen

  • Differential Analysis of Fecal SCFAs and Their Contribution to Adipogenesis in UCP1 Knock-In Pigs

    作者：Zhao, Chengyu;Huang, Jiaojiao;Zhao, Chengyu;Huang, Jiaojiao;Zhao, Chengyu;Pan, Jianfei;Wang, Yanfang;Zhao, Jianguo

    关键词：pigs; UCP1; SCFAs; caproic acid; fat deposition; gut microbiota

  • Establishment and identification of spleen cell line from tiger puffer fish (Takifugu rubripes) and immune transcriptome response to IHNV

    作者：He, Yangbin;Zhang, Jingjing;Meng, Bo;He, Yangbin;Zhang, Jingjing;Lin, Lei;Meng, Bo;Dong, Caichao;Liu, Yuyan;Yang, Yu;Ji, Honglong;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei

    关键词：Tiger puffer fish; Spleen; Cell line; Immunology; Transcriptome

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

微信小程序