Comparative transcriptome, ultrastructure and histology analyses provide insights into the potential mechanism of growth arrest in south China carp (Cyprinus carpio rubrofuscus)

文献类型： 外文期刊

第一作者： Zhong, Zaixuan

作者： Zhong, Zaixuan;Fan, Jiajia;Tian, Yuanyuan;Zhu, Huaping;Ma, Dongmei;Zhong, Zaixuan;Fan, Jiajia;Tian, Yuanyuan;Zhu, Huaping;Ma, Dongmei;Zhong, Zaixuan;Fan, Jiajia;Tian, Yuanyuan;Zhu, Huaping;Ma, Dongmei

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关键词： South China carp; Growth arrest; RNA-seq; Ultrastructure; Histology

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

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收录情况： SCI

摘要： BackgroundSouth China carp (Cyprinus carpio rubrofuscus), which is an economically important species, is traditionally cocultured with rice. Our previous study indicated that approximately 10-30% of these fish experienced growth arrest, severely impacting production. However, the molecular mechanism underlying growth inhibition in south China carp is currently unknown.ResultsIn this study, we compared the transcriptomes of the livers, muscles and intestines of carp in the fast-growing and slow-growing groups. We identified 2182, 2355 and 916 differentially expressed genes (DEGs), respectively. In the slow-growing group, the oxidative phosphorylation pathway was significantly upregulated in the liver. Transmission electron microscopy (TEM) confirmed mitochondrial damage in the liver, which was characterized by broken cristae and heterogeneous matrix. Additionally, analysis of antioxidant enzyme and transaminase activity also revealed that the livers in slow-growing individuals were unhealthy. In muscle tissue, the mitophagy and autophagy pathways were significantly dysregulated. Consequently, manifestations of mitochondrial damage and sparse myofilaments were clearly observed in slow-growing south China carp via TEM. Furthermore, pathways that regulate cell proliferation and migration, including the ECM receptor and focal adhesion, were significantly enriched in the intestine. Morphological examination revealed that the villus height and muscular layer height in the slow-growing group were significantly shorter than those in the fast-growing group, suggesting decreased intestinal cell motility. Overall, our study elucidated mitochondrial damage in the liver and muscle and detected morphological changes in intestinal villi.ConclusionsIn summary, our results help elucidate the genetic architecture related to growth arrest in south China carp and provide a basis for further research on the growth of teleosts.

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