An alfalfa MYB-like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3-mediated degradation
文献类型： 外文期刊
第一作者： Shi, Kun
作者： Shi, Kun;Liu, Jia;Dong, Hongbin;Zhang, Jinli;Wei, Yuanhong;Zhou, Le;Wang, Shaopeng;Zhu, Jiahao;Wang, Zan;Liang, Huan;Cao, Mingshu;Jones, Chris S.;Ma, Dongmei
作者机构：
关键词： alfalfa; drought resistance; E3 ubiquitin ligase; MsMYBH; MsWAV3; MYB-like transcriptional factor
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）
ISSN： 1672-9072
年卷期： 2024 年 66 卷 4 期
页码：
收录情况： SCI
摘要： Drought is a major threat to alfalfa (Medicago sativa L.) production. The discovery of important alfalfa genes regulating drought response will facilitate breeding for drought-resistant alfalfa cultivars. Here, we report a genome-wide association study of drought resistance in alfalfa. We identified and functionally characterized an MYB-like transcription factor gene (MsMYBH), which increases the drought resistance in alfalfa. Compared with the wild-types, the biomass and forage quality were enhanced in MsMYBH overexpressed plants. Combined RNA-seq, proteomics and chromatin immunoprecipitation analysis showed that MsMYBH can directly bind to the promoters of MsMCP1, MsMCP2, MsPRX1A and MsCARCAB to improve their expression. The outcomes of such interactions include better water balance, high photosynthetic efficiency and scavenge excess H2O2 in response to drought. Furthermore, an E3 ubiquitin ligase (MsWAV3) was found to induce MsMYBH degradation under long-term drought, via the 26S proteasome pathway. Furthermore, variable-number tandem repeats in MsMYBH promoter were characterized among a collection of germplasms, and the variation is associated with promoter activity. Collectively, our findings shed light on the functions of MsMYBH and provide a pivotal gene that could be leveraged for breeding drought-resistant alfalfa. This discovery also offers new insights into the mechanisms of drought resistance in alfalfa.
分类号：
- 相关文献
作者其他论文 更多>>
-
Oral delivery of miR-146a-5p overexpression plasmid-loaded Pickering double emulsion modulates intestinal inflammation and the gut microbe
作者：Zhu, Jiahao;Fan, Yaotian;Yang, Songfeng;Qin, Mengran;Luo, Junyi;Chen, Ting;Sun, Jiajie;Zhang, Yongliang;Xi, Qianyun;Chen, Xingping
关键词：Inflammatory injury; miR-146a-5p; Pickering double emulsion; Intestinal homeostasis; Lactobacillus reuteri
-
Massively Parallel CRISPR-Cas9 Knockout Screening in Sheep Granulosa Cells for FSH Response Genes
作者：Liu, Zaixia;Dai, Lingli;Bao, Yanchun;Gu, Mingjuan;Shi, Caixia;Zhou, Le;Ma, Fengying;Na, Risu;Zhang, Wenguang;Liu, Zaixia;Dai, Lingli;Bao, Yanchun;Gu, Mingjuan;Guo, Lili;Zhou, Le;Ma, Fengying;Na, Risu;Zhang, Wenguang;Dai, Lingli;Sun, Tianhao;Liu, Yongbin;Fu, Shaoyin;He, Xiaolong;Wang, Yu;Guo, Lili;Zhang, Wenguang
关键词：granulosa cells; FSH; massively parallel CRISPR-Cas9 library; dose-dependent genes
-
Effects of Continuous Cropping and Application of Bio-organic Fertilizers on Growth, Yield and Quality of Sugar Beet under Reduced Chemical Fertilizer Application
作者：Guo, Xiaoxia;Song, Baiquan;Zhou, Jianchao;Guo, Xiaoxia;Tian, Lu;Li, Yinghao;Huang, Chunyan;Li, Zhi;Jian, Caiyuan;Han, Kang;Su, Wenbin;Zhang, Peng;Kong, Dejuan;Wang, Zhenzhen;Liu, Jia
关键词：Sugar beet; Continuous cropping; Bio-organic fertilizer; Growth; Yield; Sugar content
-
Higher stochasticity in comammox Nitrospira community assembly in upland soils than the adjacent paddy soils at a regional scale
作者：Feng, Mengmeng;Lin, Yongxin;Jin, Shengsheng;Wan, Song;Cheng, Yuheng;He, Ji-Zheng;He, Zi-Yang;Hu, Hang-Wei;He, Ji-Zheng;Liu, Jia
关键词：Agricultural soils; Comammox Nitrospira; Community assembly; Nitrification; Stochastic processes
-
Frequencies of insecticide resistance mutations detected by the amplicon sequencing in Plutella xylostella (Lepidoptera: Plutellidae) and Spodoptera exigua (Lepidoptera: Noctuidae) from China
作者：Liu, Zhangyang;Ma, Haihao;Li, Kaiqin;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Liu, Zhangyang;Ma, Haihao;Li, Kaiqin;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Liu, Zhangyang;Li, Kaiqin;Zhou, Xiaomao
关键词：Plutella xylostella; Spodoptera exigua; insecticide resistance; mutation detection; amplicon sequencing
-
The Application of Rice Straw with Reduced N Fertilizer Improves the Rice Yield While Decreasing Environmental N Losses in Southern China
作者：Liu, Han;Ma, Tingting;Wan, Li;Chen, Xiaofen;Liu, Jia;Liu, Han;Ma, Tingting;Wan, Li;Chen, Xiaofen;Liu, Jia;Liu, Han;Zhou, Guopeng;Zhu, Anfan
关键词：greenhouse gases; NH3 volatilization; net ecosystem carbon budget; rice yield
-
Integrative analysis of the ovarian metabolome and transcriptome of the Yaoshan chicken and its improved hybrids
作者：Miao, Xiaomeng;Li, Liang;Miao, Xiaomeng;Zhang, Yalan;Ning, Zhonghua;Wu, Tian;Pan, Hongyuan;Huang, Tengda;Liu, Jia;Fan, Ying;Du, Lin;Gong, Yu
关键词：egg production; ovary; chicken; transcriptome; metabolomics
