Inactivated Rabies Virus Vectored MERS-Coronavirus Vaccine Induces Protective Immunity in Mice, Camels, and Alpacas

文献类型： 外文期刊

第一作者： Chi, Hang

作者： Chi, Hang;Li, Entao;Han, Qiuxue;Wang, Zhenshan;Wang, Xinyue;Feng, Na;Gao, Yuwei;Zhao, Yongkun;Yang, Songtao;Xia, Xianzhu;Wang, Yanqun;Zhu, Airu;Sun, Jing;Zhuang, Zhen;Zhao, Jincun;Wang, Xiwen;Wang, Hualei;Jin, Hongli;Zhang, Lu;Ye, Jingmeiqi;Wang, Haijun;Hu, Mingda;Zhao, Jincun;Zhao, Jincun

作者机构：

关键词： MERS-CoV; vaccine; rabies virus vector; mice; camel; alpaca

期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：8.786； 五年影响因子：8.876 ）

ISSN： 1664-3224

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Middle East respiratory syndrome coronavirus (MERS-CoV) is an emergent coronavirus that has caused frequent zoonotic events through camel-to-human spillover. An effective camelid vaccination strategy is probably the best way to reduce human exposure risk. Here, we constructed and evaluated an inactivated rabies virus-vectored MERS-CoV vaccine in mice, camels, and alpacas. Potent antigen-specific antibody and CD8(+) T-cell responses were generated in mice; moreover, the vaccination reduced viral replication and accelerated virus clearance in MERS-CoV-infected mice. Besides, protective antibody responses against both MERS-CoV and rabies virus were induced in camels and alpacas. Satisfyingly, the immune sera showed broad cross-neutralizing activity against the three main MERS-CoV clades. For further characterization of the antibody response induced in camelids, MERS-CoV-specific variable domains of heavy-chain-only antibody (VHHs) were isolated from immunized alpacas and showed potent prophylactic and therapeutic efficacies in the Ad5-hDPP4-transduced mouse model. These results highlight the inactivated rabies virus-vectored MERS-CoV vaccine as a promising camelid candidate vaccine.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序