NAC transcription factor SlNOR-like1 plays a dual regulatory role in tomato fruit cuticle formation

文献类型： 外文期刊

第一作者： Liu, Gang-Shuai

作者： Liu, Gang-Shuai;Ji, Xiang;Peng, Zhen-Zhen;Li, Hong-Li;Niu, Xiao-Lin;Jia, Wen;He, Jian-Lin;Xiang, Lan-Ting;Qu, Gui-Qin;Zhu, Hong-Liang;Zhu, Ben-Zhong;Luo, Yun-Bo;Fu, Da-Qi;Huang, Hua;Huang, Hua;Grierson, Donald;Gao, Ying;Grierson, Donald;Gao, Ying;Gao, Hai-Yan

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关键词： Cuticle; cutin; SlNOR-like1; tomato fruit; transcription factor; wax

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The plant cuticle is an important protective barrier on the plant surface, constructed mainly by polymerized cutin matrix and a complex wax mixture. Although the pathway of plant cuticle biosynthesis has been clarified, knowledge of the transcriptional regulation network underlying fruit cuticle formation remains limited. In the present work, we discovered that tomato fruits of the NAC transcription factor SlNOR-like1 knockout mutants (nor-like1) produced by CRISPR/Cas9 [clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9] displayed reduced cutin deposition and cuticle thickness, with a microcracking phenotype, while wax accumulation was promoted. Further research revealed that SlNOR-like1 promotes cutin deposition by binding to the promoters of glycerol-3-phosphate acyltransferase6 (SlGPAT6; a key gene for cutin monomer formation) and CUTIN DEFICIENT2 (SlCD2; a positive regulator of cutin production) to activate their expression. Meanwhile, SlNOR-like1 inhibits wax accumulation, acting as a transcriptional repressor by targeting wax biosynthesis, and transport-related genes 3-ketoacyl-CoA synthase1 (SlKCS1), ECERIFERUM 1-2 (SlCER1-2), SlWAX2, and glycosylphosphatidylinositol-anchored lipid transfer protein 1-like (SlLTPG1-like). In conclusion, SlNOR-like1 executes a dual regulatory effect on tomato fruit cuticle development. Our results provide a new model for the transcriptional regulation of fruit cuticle formation. In tomato fruit, SlNOR-like1 promotes cutin deposition by targeting and activating SlGPAT6 and SlCD2 . Meanwhile, SlNOR-like1 inhibits wax accumulation by targeting and inhibiting SlKCS1 , SlCER1-2 , SlWAX2 , and SlLTPG1-like .

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