Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells

文献类型： 外文期刊

第一作者： Yu, Huahong

作者： Yu, Huahong;Li, Xiangmei;Liang, Zhongshang;Luo, Ting;Li, Jing;Li, Hongyan;Deng, Zeyuan;Qiu, Bin;Li, Siguang

作者机构：

期刊名称：JOURNAL OF CHEMISTRY （ 影响因子：2.506； 五年影响因子：2.726 ）

ISSN： 2090-9063

年卷期： 2017 年

页码：

收录情况： SCI

摘要： Researches have demonstrated that trans-fatty acids are related to the progression of atherosclerosis, but the underlying mechanism is not clear till now. In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. In human umbilical vein endothelial cells (HUVEC), twenty-two and twenty-three differentially expressed proteins were identified in low (50 mu mol/L) and high (400 mu mol/L) concentration elaidic acid simulated groups, respectively, comparing with the control group. The expressions of some selected proteins (PSME3, XRCC5, GSTP(1), andGSTO(1)) were validated by qRT-PCR analysis. Western blotting analysis further confirmed that elaidic acid downregulated the expression of PSME3 and XRCC5. Moreover, P53, the downstream protein of PSME3, was further investigated. Results demonstrated that a variety of proteins, many of which were related to oxidative stress, apoptosis, and DNA damage, were involved in the elaidic acid induced atherosclerosis. Furthermore, P53 was demonstrated to regulate the atherosclerosis through cell cycle arrest and apoptosis pathway.

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