Silencing of the T-type voltage-gated calcium channel α1 subunit by fungus-mediated RNAi altered the structure of F-actin and caused defective behaviors in Ditylenchus destructor

文献类型： 外文期刊

第一作者： Yang, Jiahao

作者： Yang, Jiahao;Zhou, Siyu;Yang, Ziqi;Shi, Xuqi;Liu, Haoran;Yang, Zhuhong;Ding, Zhong;Ye, Shan;Yang, Zhuhong;Ding, Zhong;Ye, Shan;Peng, Deliang

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关键词： T-type voltage-gated calcium channels (VGCCs); Ditylenchus destructor; Plant parasitic nematodes; Physiological function; F-actin

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2024 年 51 卷 1 期

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收录情况： SCI

摘要： Background T-type calcium channels, characterized as low-voltage activated (LVA) calcium channels, play crucial physiological roles across a wide range of tissues, including both the neuronal and nonneuronal systems. Using in situ hybridization and RNA interference (RNAi) techniques in vitro, we previously identified the tissue distribution and physiological function of the T-type calcium channel alpha 1 subunit (DdC alpha 1G) in the plant-parasitic nematode Ditylenchus destructor. Methods and results To further characterize the functional role of DdC alpha 1G, we employed a combination of immunohistochemistry and fungus-mediated RNAi and found that DdC alpha 1G was clearly distributed in stylet-related tissue, oesophageal gland-related tissue, secretory-excretory duct-related tissue and male spicule-related tissue. Silencing DdC alpha 1G led to impairments in the locomotion, feeding, reproductive ability and protein secretion of nematodes. To confirm the defects in behavior, we used phalloidin staining to examine muscle changes in DdC alpha 1G-RNAi nematodes. Our observations demonstrated that defective behaviors are associated with related muscular atrophy. Conclusion Our findings provide a deeper understanding of the physiological functions of T-type calcium channels in plant-parasitic nematodes. The T-type calcium channel can be considered a promising target for sustainable nematode management practices.

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