Identification of three novel linear B-cell epitopes on VP7 of African horse sickness virus using monoclonal antibodies

文献类型： 外文期刊

第一作者： Hu, Xinbing

作者： Hu, Xinbing;Xu, Jing;Wang, Xuanying;Tian, Zhancheng;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Du, Junzheng;Hu, Xinbing;Xu, Jing;Wang, Xuanying;Tian, Zhancheng;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Du, Junzheng;Yin, Hong

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关键词： African horse sickness virus; Soluble VP7; Monoclonal antibodies; Three B-cell epitopes

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2024 年 298 卷

页码：

收录情况： SCI

摘要： African horse sickness (AHS) is an acute and subacute infectious disease of equine species caused by the African horse sickness virus (AHSV). The VP7 of AHSV is a group-specific protein conserved in all serotypes and is an excellent candidate for the serological diagnosis and an AHS vaccine component. However, to date, B-cell epitopes on the AHSV VP7 recognized by humoral immune responses remain unclear. This study expressed the recombinant AHSV VP7 soluble in Escherichia coli and purified it for mouse immunization. Four monoclonal antibodies (mAbs) were screened and identified by hybridoma cell fusion, clonal purification, and immunological assays. The B-cell epitopes, recognized by monoclonal antibodies 4B5, 3G10, 3D7, and 4D6, were identified by a series of truncated overlapping peptides expressed as glutathione S-transferase (GST)-fusion proteins. The results revealed that 4B5 recognized the (124)VQTGRYAGA(132) motif, 3G10 recognized the (140)RYYVPQGRT(148) motif, while 3D7 and 4D6 recognized the (292)QPINPPIFP(300) motif. Amino acid sequence alignment indicated that three novel B-cell epitopes were conserved among various AHSV serotypes but unconserved in other orbiviruses, such as the bluetongue and epidemic hemorrhagic disease viruses. This study informs on the antigenic epitopes of AHSV VP7, facilitating future investigations into the serological diagnosis method and epitope-based vaccines against AHSV.

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