Long-term, multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production

文献类型： 外文期刊

第一作者： Fan, Ziyao

作者： Fan, Ziyao;Liu, Zhiguo;Xu, Kui;Wu, Tianwen;Ruan, Jinxue;Mu, Yulian;Li, Kui;Zheng, Xinmin;Bao, Shideng;Sonstegard, Tad

作者机构：

关键词： MSTN-edited pigs; multidomain evaluation; genetic background; editing strategy

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：6.038； 五年影响因子：4.754 ）

ISSN： 1674-7305

年卷期：

页码：

收录情况： SCI

摘要： Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin (MSTN), but a lack of diversity in the MSTN germplasm has limited similar advances in pig farming. Moreover, insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion, reproduction, and meat quality in MSTN-edited pigs have also currently blocked progress. Here, in a largest-to-date evaluation of multiple MSTN-edited pig populations, we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb weakness. We also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter size. Apart from establishing a new benchmark for the data scale and quality of genome-edited animal production, our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic backgrounds.

分类号：

  • 相关文献
作者其他论文 更多>>
  • A rapid and highly sensitive CRISPR assay utilizing Cas12a orthologs for the detection of Novel Duck Reovirus

    作者：Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Yuan;Fu, Lanting;Li, Sheng;Tao, Dagang;Ruan, Jinxue;Xie, Shengsong;Wang, Cui;He, Daqian;Gong, Ping;Yang, Yu

    关键词：NDRV; Cas12a orthologs; Gs12-16; Gs12-18; CRISPR-based diagnostics

  • EXPERT expands prime editing efficiency and range of large fragment edits

    作者：Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Han, Xiaosong;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Li, Kui;Zhang, Jifeng;Xu, Jie

    关键词：

  • Molecular pathological characteristics and mechanisms of the liver in metabolic disease-susceptible transgenic pigs

    作者：Du, Juan;Zhang, Kaiyi;Miao, Jiakun;Yang, Yu;Tian, Yuying;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Yang, Shulin

    关键词：Transgenic pigs; ETNPPL; GABBR2; BMP8B; Bis (monoacylglycerol) phosphates; Sulfatides

  • Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

    作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang

    关键词：cis-regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq

  • Transcriptome analysis reveals no obvious unintended effects in the spleen of CRISPR/Cas9-mediated CD163 and pAPN double-knockout pigs

    作者：Liu, Zhi-Guo;Wang, Nan;Wang, Wan-Jie;Chi, Shun-Shun;Nie, Yu-Xin;Yuan, Mao-Sha;Sun, Ya-Ru;Mu, Yu-Lian;Li, Kui;Wang, Nan;Huang, Lei;Chi, Shun-Shun;Li, Kui

    关键词：CD163; pAPN; Double-knockout; Gene editing; Pig; Spleen; Unintended effects; Transcriptomics

  • Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs

    作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang

    关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits

  • Single-nucleus RNA sequencing defines adipose tissue subpopulations that contribute to Tibetan pig cold adaptation

    作者：Liu, Jiali;Chen, Chuanhe;Chui, Linya;Zhang, Xueping;Wang, Xiao;Yang, Chunhuai;Wu, Tianwen;Yang, Shulin;Tao, Cong;Wang, Yanfang;Jiang, Yao;Liu, Tianxia;Cui, Along;Zhang, Xueping;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Liu, Tianxia;Wang, Yu;Zhao, Jianguo;Liu, Tianxia;Cui, Along;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Zhang, Ying;Zhao, Jianguo;Cui, Along;Zhang, Xueping;Chui, Linya;Huang, Jiaojiao;Zhao, Jianguo;Wang, Yanfang

    关键词：Pig; snRNA-seq; Adipocytes; Subpopulations; Cold stimulation

微信小程序