Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

文献类型： 外文期刊

第一作者： Lodi, Rathna Silviya

作者： Lodi, Rathna Silviya;Dong, Xiaodan;Jiang, Chunhui;Sun, Zhou;Deng, Peng;Sun, Sujun;Wang, Xinkun;Wang, Hengzhen;Peng, Lizeng;Peng, Chune;Jiang, Chunhui;Huang, Xiaopeng;Sun, Zhou;Mesa, Arundathi

作者机构：

关键词： antimicrobial activity; Codonopsis pilosula; endophytes; enzymatic activity; secondary metabolites

期刊名称：FEMS MICROBIOLOGY ECOLOGY （ 影响因子：4.2； 五年影响因子：5.0 ）

ISSN： 0168-6496

年卷期： 2023 年 99 卷 8 期

页码：

收录情况： SCI

摘要： Endophytes of Codonopsis pilosula and their potentiality to produce antimicrobial secondary metabolites and enzymes. The roots of the medicinal plant Codonopsis pilosula (Franch.) Nannf (C. pilosula) possess most medicinal supplements. In current research on C. pilosula root endophytes were isolated, identified, and evaluated for their antimicrobial activity against human pathogens such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Pseudomonas aeruginosa and the fungi Candida albicans and Aspergillus niger. Endophytes C.P-8 and C.P-20 exhibited very significant antimicrobial activity, the secondary metabolite of C.P-8 registered at retention time 24.075 by HPLC analysis. Significant minimum inhibitory concentration (MIC) of C.P-8 was exhibited at 250 & mu;g/ml against S. aureus and 500 & mu;g/ml against B. subtilis. Qualitative, quantitative analyses, and partial purification of enzymes and purity was analysed by molecular weight determined by SDS-PAGE of enzymes produced by C.P-20, amylase-64 kDa, protease-64 kDa, chitinase-30 kDa, and cellulase-54 kDa. Optimum pH and temperature of the partially purified enzymes, was carried out. The partially purified enzymes from C.P-20 displayed maximum activity at pH 6-7 and temperatures of 40 & DEG;C-45 & DEG;C. Moreover, the above endophytes will be useful tools for producing active enzymes and active bioantimicrobial agents against human pathogens.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序