An immune-enhanced multivalent DNA nanovaccine to prevent H7 and H9 avian influenza virus in mice

文献类型： 外文期刊

第一作者： Xu, Shangen

作者： Xu, Shangen;Jin, Zheng;Qu, Yang;Li, Jiawei;Zhang, Qihong;Zhao, Kai;Lan, Hailing;Teng, Qiaoyang;Li, Xuesong;Li, Zejun;Lan, Hailing;Qu, Yang;Kang, Hong;Zhao, Kai;Lan, Hailing;Qu, Yang;Kang, Hong;Zhao, Kai;Jin, Zheng;Yin, Tan Hui;Zhao, Kai;Yin, Tan Hui

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关键词： H7 AIV; Dendrigraft poly-l-lysines; Nanoparticles; Multivalent DNA nanovaccine

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 251 卷

页码：

收录情况： SCI

摘要： H7 avian influenza virus has caused multiple human infections and poses a severe public health threat. In response to the highly variable nature of AIVs, a novel, easily regenerated DNA vaccine has great potential in treating or preventing avian influenza pandemics. Nevertheless, DNA vaccines have many disadvantages, such as weak immunogenicity and poor in vivo delivery. To further characterize and solve these issues and develop a novel H7 AIV DNA vaccine with enhanced stability and immunogenicity, we constructed nine AIV DNA plasmids, and the immunogenicity screened showed that mice immunized with pl3H7N2SH9 elicited stronger hemagglutination-inhibiting (HI) antibodies than other eight plasmid DNAs. Then, to address the susceptibility to degradation and low transfection rate of DNA vaccine in vivo, we developed pl3H7N2SH9/DGL NPs by encapsulating the pl3H7N2SH9 within the dendrigraft poly-l-lysines nanoparticles. As expected, these NPs exhibited excellent physical and chemical properties, were capable of promote lymphocyte proliferation, and induce stronger humoral and cellular responses than the naked pl3H7N2SH9, including higher levels of HI antibodies than naked pl3H7N2SH9, as well as the production of cytokines, namely, IL-2, IFN-& alpha;. Taken together, our results suggest that the construction of an immune-enhanced H7-AIV DNA nanovaccine may be a promising strategy against most influenza viruses.

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