Chromosome 5P of Agropyron cristatum induces chromosomal translocation by disturbing homologous chromosome pairing in a common wheat background

文献类型： 外文期刊

第一作者： Han, Haiming

作者： Han, Haiming;Ma, Xinyuan;Wang, Zhen;Qi, Kai;Yang, Wenjing;Liu, Weihua;Zhang, Jinpeng;Zhou, Shenghui;Lu, Yuqing;Yang, Xinming;Li, Xiuquan;Li, Lihui

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关键词： Agropyron cristatum; Wheat; Chromosome 5P; Translocation; Chromosome pairing

期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）

ISSN： 2095-5421

年卷期： 2023 年 11 卷 1 期

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收录情况： SCI

摘要： Wide hybridization is a strategy for broadening the genetic basis of wheat. Because an efficient method for inducing wheat-alien chromosome translocations will allow producing useful germplasm, it is desir-able to discover new genes that induce chromosomal variation. In this study, chromosome 5P from A. cristatum was shown to induce many types of chromosomal structural variation in a common wheat background, including nonhomoeologous chromosome translocations, as revealed by genomic in situ hybridization, fluorescence in situ hybridization, and DNA marker analysis. Aberrant meiosis was associ-ated with chromosomal structural variation, and aberrant meiotic behavior was observed in wheat-A. cristatum 5P monosomic and disomic addition lines, suggesting that the effect of chromosome 5P was independent of the number of chromosome 5P copies. Chromosome 5P disturbed homologous chromo-some pairing at pachytene stage in a common wheat background, resulting in a high frequency of univa-lent formation and reduced crossing over. Thirteen genes involved in DNA repair or chromatin remodeling, including RAD52-like and MSH6 genes, were differentially expressed (upregulated) in wheat-A. cristatum 5P addition lines according to transcriptome analysis, implicating chromosome 5P in the process of meiotic double-strand break repair. These findings provide a new, efficient tool for inducing wheat-alien chromosome translocations and producing new germplasm.(c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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