文献类型： 外文期刊
第一作者： Fu, Huanru
作者： Fu, Huanru;Chen, Shuyu;Zhang, Jinpeng;Su, Jinbo;Miao, Zhongwei;Huang, Yu;Wan, Chunhe;Fu, Huanru;Chen, Shuyu;Su, Jinbo
作者机构：
关键词： goose megrivirus; TaqMan real-time PCR technology; epidemiological investigation; vertical; transmission
期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0032-5791
年卷期： 2024 年 103 卷 5 期
页码：
收录情况： SCI
摘要： The aim of this study was to develop an efficient and accurate platform for the detection of the newly identified goose megrivirus (GoMV). To achieve this goal, we developed a TaqMan real-time PCR technology for the rapid detection and identifica- tion of GoMV. Our data showed that the established TaqMan real-time PCR assay had high sensitivity, with the lowest detection limit of 67.3 copies/mL. No positive signal can be observed from other goose origin viruses (including AIV, GPV, GoCV, GHPyV, and GoAstV), with strong specificity. The coefficients of variation of repeated intragroup and intergroup tests were all less than 1.5%, with excellent repeatability. Clinical sample investigation data from domestic Minbei White geese firstly provided evidence that GoMV can be transmitted both horizontally and vertically. In conclusion, since the TaqMan real-time PCR method has high sensitivity, specificity, and reproducibility, it can be a useful candidate tool for GoMV epidemiological investigation
分类号：
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification, Sequence Alignment, and Transcription of Five Sex-Related Genes in Largemouth Bass (Micropterus Salmoides)
作者：Zhang, Xinhui;You, Xinxin;Liu, Huan;Liu, Xin;Shi, Qiong;Zhang, Xinhui;Ruan, Zhiqiang;Huang, Yu;You, Xinxin;Wang, Xinwen;Xu, Junmin;Shi, Qiong;Sun, Chengfei;Ye, Xing;Hu, Cancan;Wang, Xinwen;Xu, Junmin;Liu, Huan;Liu, Xin;Shi, Qiong
关键词：largemouth bass; sex-related gene; transcriptome sequencing; gonadal development; differentially expressed genes
-
Pre-breeding of spontaneous Robertsonian translocations for density planting architecture by transferring Agropyron cristatum chromosome 1P into wheat
作者：Han, Bohui;Wang, Xiao;Kang, Xilu;Zhang, Meng;Luo, Jiawen;Han, Haiming;Zhou, Shenghui;Lu, Yuqing;Liu, Weihua;Yang, Xinming;Li, Xiuquan;Zhang, Jinpeng;Li, Lihui;Zhang, Jinpeng;Li, Lihui;Zhang, Jinpeng;Li, Lihui;Sun, Yangyang
关键词：
-
Duck compound probiotics fermented diet alters the growth performance by shaping the gut morphology, microbiota and metabolism
作者：Li, Zhaolong;Li, Cuiting;Lin, Fengqiang;Yan, Lu;Wu, Huini;Zhou, Haiou;Guo, Qing;Huang, Yu;Lin, Binbin;Xie, Bilin;Xu, Yijuan;Lin, Zhimin;Liu, Wenjin
关键词：fermented diet; duck; compound probiotics; growth performance
-
First Report of a Novel Goose Adenovirus Outbreak in Lion Head Gooses in China
作者：Liu, Rongchang;Lan, Qin;Zhang, Jiaxue;Liang, Qizhang;Fu, Guanghua;Huang, Yu;Liu, Rongchang;Liao, Ming;Sun, Minhua;Liao, Ming;Sun, Minhua;Liao, Ming
关键词：
-
A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost
作者：Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Li, Yuan;Zhang, Defeng;Qiang, Jun
关键词：Nile tilapia; Endothelial cell line; BBB; Astrocyte-conditioned medium (ACM)
-
QTL mapping for agronomic traits in Agropyron Gaertn. CP population at the flowering stage
作者：Che, Yonghe;Yang, Jing;He, Yutong;Song, Nan;Yang, Yanping;Wei, Lai;Che, Yonghe;Yang, Jing;He, Yutong;Song, Nan;Yang, Yanping;Wei, Lai;Yang, Xinming;Zhang, Yannong;Zhang, Jinpeng;Han, Haiming;Li, Xiuquan;Zhou, Shenghui;Liu, Weihua;Li, Lihui
关键词：Agropyron Gaertn.; CP hybrid population; Flowering stage; Major QTL
-
Codon usage bias of goose circovirus and its adaptation to host
作者：Xu, Quanming;Cao, Jie;Wan, Chunhe;Rai, Kul Raj;Zhu, Binling;Liu, Dan
关键词：goose circovirus; codon usage bias; natural selection; mutation; host adaptability
