Rapid detection of goose megrivirus using TaqMan real-time PCR technology

文献类型： 外文期刊

第一作者： Fu, Huanru

作者： Fu, Huanru;Chen, Shuyu;Zhang, Jinpeng;Su, Jinbo;Miao, Zhongwei;Huang, Yu;Wan, Chunhe;Fu, Huanru;Chen, Shuyu;Su, Jinbo

作者机构：

关键词： goose megrivirus; TaqMan real-time PCR technology; epidemiological investigation; vertical; transmission

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 5 期

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收录情况： SCI

摘要： The aim of this study was to develop an efficient and accurate platform for the detection of the newly identified goose megrivirus (GoMV). To achieve this goal, we developed a TaqMan real-time PCR technology for the rapid detection and identifica- tion of GoMV. Our data showed that the established TaqMan real-time PCR assay had high sensitivity, with the lowest detection limit of 67.3 copies/mL. No positive signal can be observed from other goose origin viruses (including AIV, GPV, GoCV, GHPyV, and GoAstV), with strong specificity. The coefficients of variation of repeated intragroup and intergroup tests were all less than 1.5%, with excellent repeatability. Clinical sample investigation data from domestic Minbei White geese firstly provided evidence that GoMV can be transmitted both horizontally and vertically. In conclusion, since the TaqMan real-time PCR method has high sensitivity, specificity, and reproducibility, it can be a useful candidate tool for GoMV epidemiological investigation

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