Protective Effect of Phloretin against Hydrogen Peroxide-Induced Oxidative Damage by Enhancing Autophagic Flux in DF-1 Cells

文献类型： 外文期刊

第一作者： Song, Dan

作者： Song, Dan;Liu, Feng;Tao, Wenjing;Wu, Xian;Bi, Haiyang;Li, Xiangchen;Song, Dan;Liu, Feng;Tao, Wenjing;Wu, Xian;Bi, Haiyang;Li, Xiangchen;Shu, Jianhong;Shu, Jianhong;Wang, Dong

作者机构：

期刊名称：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY （ 影响因子：7.31； 五年影响因子：8.427 ）

ISSN： 1942-0900

年卷期： 2022 年 2022 卷

页码：

收录情况： SCI

摘要： Phloretin (PHL) is a dihydrochalcone flavonoid isolated from the peel and root bark of apples, strawberries, and other plants with antioxidative characteristic. In this study, we aimed to investigate the protective effect and the potential mechanism of PHL on hydrogen peroxide (H2O2)-induced oxidative damage in DF-1 cells. The results showed that PHL exhibited no cytotoxic effect on DF-1 cells at concentration below 20 mu M. PHL markedly increased H2O2-reduced cell viability, decreased H2O2-induced apoptosis, as evidenced by reduced apoptosis rate, the upregulation of gene and protein level of Bcl-2, and the downregulation of gene and protein level of Bax and Cleaved caspase3. In addition, PHL reduced H2O2-induced reactive oxygen species (ROS) production and restored antioxidant enzymes activities as well as mitochondrial membrane potential in a dose-dependent manner. Moreover, PHL prior to H2O2 further increased LC3-II level, promoted p62 turnover and improved lysosomal function. Importantly, autophagy inhibitor chloroquine (CQ) reversed the protective effect of PHL, and increased H2O2-induced apoptosis. Furthermore, PHL inhibited the phosphorylation levels of ERK, p38, and JNK. Collectively, these results indicate that PHL could attenuate H2O2-induced oxidative injury and apoptosis by maintaining lysosomal function and promoting autophagic flux, and MAPKs pathway may be involved in this process. Our study provides evidence that PHL could as a new strategy to against oxidative damage in poultry industry.

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