An S-locus F-box protein as pollen S determinant targets non-self S-RNase underlying self-incompatibility in Citrus

文献类型： 外文期刊

第一作者： Cao, Zong-Hong

作者： Cao, Zong-Hong;Song, Dan;Hu, Yu;Liang, Mei;Xu, Qiang;Ye, Jun-Li;Xie, Zong-Zhou;Deng, Xiu-Xin;Chai, Li-Jun;Wang, Shao-Hua;Deng, Xiu-Xin;Chai, Li-Jun

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关键词： Citrus; non-self recognition; SCF complex; pollen S determinants; SI; SLF

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Self-incompatibility (SI) is a crucial mechanism that prevents self-fertilization and inbreeding in flowering plants. Citrus exhibits SI regulated by a polymorphic S-locus containing an S-RNase gene and multiple S-locus F-box (SLF) genes. It has been documented that S-RNase functions as the pistil S determinant, but there is no direct evidence that the SLF genes closely linked with S-RNase function as pollen S determinants in Citrus. This study assembled the genomes of two pummelo (Citrus grandis) plants, obtained three novel complete and well-annotated S-haplotypes, and isolated 36 SLF or SLF-like alleles on the S-loci. Phylogenetic analysis of 138 SLFs revealed that the SLF genes were classified into 12 types, including six types with divergent or missing alleles. Furthermore, transformation experiments verified that the conserved S-6-SLF7a protein can lead to the transition of SI to self-compatibility by recognizing non-self S8-RNase in 'Mini-Citrus' plants (S7S8 and S8S29, Fortunella hindsii), a model plant for citrus gene function studies. In vitro assays demonstrated interactions between SLFs of different S haplotypes and the Skp1-Cullin1-F-box subunit CgSSK1 protein. This study provides direct evidence that SLF controls the pollen function in Citrus, demonstrating its role in the 'non-self recognition' SI system.

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