Knockout of ABC Transporter ABCG4 Gene Confers Resistance to Cry1 Proteins in Ostrinia furnacalis

文献类型： 外文期刊

第一作者： Gao, Qing

作者： Gao, Qing;Lin, Yaling;Jing, Dapeng;Wang, Zhenying;He, Kanglai;Bai, Shuxiong;Zhang, Yongjun;Zhang, Tiantao;Gao, Qing;Wang, Xiuping;Lin, Yaling

作者机构：

关键词： CRISPR; ABCG4; Ostrinia furnacalis; resistant; Cry1 proteins

期刊名称：TOXINS （ 影响因子：4.2； 五年影响因子：4.7 ）

ISSN：

年卷期： 2022 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Ostrinia furnacalis is an important borer on maize. Long-term and large-scale planting of transgenic corn has led O. furnacalis evolving resistance and reducing the control effect. Recently, high levels of resistance to Bt Cry1 toxins have been reported to be genetically linked to the mutation or down-regulation of ABC transporter subfamily G gene ABCG4 in O. furnacalis. In order to further determine the relationship between ABCG4 gene and the resistance to Cry1 toxins in O. furnacalis, the novel CRISPR/Cas9 genome engineering system was utilized to successfully construct ABCG4-KO knockout homozygous strain. Bioassay results indicated that an ABCG4-KO strain had a higher resistance to Cry1 proteins compared with a susceptible strain (ACB-BtS). The result indicates that the ABCG4 gene may act as a receptor of the Bt Cry1 toxin in O. furnacalis. Furthermore, the development time was significantly changed in the early stage ABCG4-KO larvae, and the population parameters were also significantly changed. In summary, our CRISPR/Cas9-mediated genome editing study presents evidence that ABCG4 gene is a functional receptor for Bt Cry1 toxins, laying the foundation for further clarification of the Bt resistance mechanism.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序