Intrauterine injection of bioengineered hydrogel loaded exosomes derived from HUCM stem cells and spermidine prominently augments the pregnancy rate in thin endometrium rats

文献类型： 外文期刊

第一作者： Lin, Xiuying

作者： Lin, Xiuying;Jin, Ningyi;Lin, Xiuying;Fang, Yanqiu;Mi, Xuguang;Fu, Jianhua;Chen, Shiling;Wu, Mengxue;Jin, Ningyi;Jin, Ningyi

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关键词： Hydrogel; Exosomes; Endometrium; Intrauterine

期刊名称：REGENERATIVE THERAPY （ 影响因子：4.3； 五年影响因子：3.9 ）

ISSN： 2352-3204

年卷期： 2024 年 27 卷

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收录情况： SCI

摘要： The endometrium is essential to the development of embryos and pregnancy. Human umbilical cord mesenchymal stem cells (HUCMSCs) are promising stem cell sources. HUCMSCs self-renew quickly and are painless to collect. Spermidine is an inherent polyamine needed for cellular and molecular processes that regulate physiology and function. HUCMSCs and spermidine (SN) may heal intrauterine adhesions. HUCMSCs were investigated for endometrial repair in rats. Composite hydrogels are used for medical exosome implantation, including their materials, properties, and embedding procedures. This study examined whether bioengineered hydrogel-loaded exosomes from HUCMSCs and spermidine prenatally improved conception rates in mice with poor endometrial lining. The data show that HUCMSC and SN provide a good experimental base for HUCMSC safety and intrauterine treatment in rats. Western blots, exosome structural analysis, pregnancy outcomes, flow cytometry, H &E staining, immunohistochemistry, and immuno fluorescence labelling found and recovered the aberrant area. HUCM-derived stem cells and spermidine-derived exosomes biophysically match. These traits strengthen and prolong endometrial function. Pregnant rats with HUCMSC and SN had thinner endometrium. Hydrogel -incorporated HEHUCMSC and SN exosomes may improve IUI in rats with thin endometrium. (c) 2024, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

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