Single-cell RNA sequencing of the Mongolia sheep testis reveals a conserved and divergent transcriptome landscape of mammalian spermatogenesis

文献类型： 外文期刊

第一作者： Tian, Yu

作者： Tian, Yu;Sun, Peng;Liu, Wen-Xiang;Shan, Li-Ying;Hu, Yan-Ting;Fan, Hai-Tao;Liu, Yong-bin;Zhou, Yang;Zhang, Teng;Sun, Peng;Shen, Wei;Liu, Yong-bin

作者机构：

关键词： developmental trajectories; mongolia Sheep; spermatogenesis; single-cell transcriptome

期刊名称：FASEB JOURNAL （ 影响因子：5.834； 五年影响因子：6.103 ）

ISSN： 0892-6638

年卷期： 2022 年 36 卷 6 期

页码：

收录情况： SCI

摘要： Spermatogenesis is a highly coordinated and complex process, and is pivotal for transmitting genetic information between mammalian generations. In this study, we investigated the conservation, differences, and biological functions of homologous genes during spermatogenesis in Mongolia sheep, humans, cynomolgus monkey, and mice using single-cell RNA sequencing technology. We compared X chromosome meiotic inactivation events in Mongolia sheep, humans, cynomolgus monkey, and mice to uncover the concerted activity of X chromosome genes. Subsequently, we focused on the dynamics of gene expression, key biological functions, and signaling pathways at various stages of spermatogenesis in Mongolia sheep and humans. Additionally, the ligand-receptor networks of Mongolia sheep and humans in testicular somatic and germ cells at different developmental stages were mapped to reveal conserved germ cell-soma communication using single-cell resolution. These datasets provided novel information and insights to unravel the molecular regulatory mechanisms of Mongolia sheep spermatogenesis and highlight conservation in gene expression during spermatogenesis between Mongolia sheep and humans, providing a foundation for the establishment of a large mammalian disease model of male infertility.

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