Analysis of DNA methylation of CD79B in MDV-infected chicken spleen

文献类型： 外文期刊

第一作者： Wang Lu-Lu

作者： Wang Lu-Lu;Zhao Chun-Fang;Zhang Hao;Lian Ling;Wang Lu-Lu;Zhao Chun-Fang;Zhang Hao;Lian Ling;Liu Chang-Jun

作者机构：

关键词： chicken; Marek's disease; Marek's disease virus; DNA methylation; gene expression

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2021 年 20 卷 11 期

页码：

收录情况： SCI

摘要： Marek's disease (MD), an immunosuppressive disease induced by Marek's disease virus (MDV), provides an ideal model for studying diseases caused by a carcinogenic virus. CD79B is a B-cell antigen receptor complex-associated protein beta-chain precursor which is involved in the activation, proliferation, differentiation of B-cell and the transmission of downstream signals. This study analyzed CD79B gene mRNA expression and methylation by two schemes #20 (5 ' flanking to intron 1) and #27 (intron 2 to intron 3), between MDV-infected tumorous spleens (TS) and non-infected spleens (NS). Results showed that average methylation levels of CpGs in #20 and #27 were higher in TS than in NS (P<0.05), while, CD79B mRNA expression was lower in TS than in NS (P<0.01). Six of 40 CpG sites showed significantly (P<0.05) different methylation levels between TS and NS. Correlation analysis showed that the average methylation level rather than a single site methylation level in #20 affected (P<0.05) mRNA expression. Collectively, it was found that the change of CD79B gene expression after MDV infection might be partly explained by modification of DNA methylation.

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