Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass

文献类型： 外文期刊

第一作者： Yang, Yibin

作者： Yang, Yibin;He, Hao;Yang, Qiuhong;Ai, Xiaohui;Zhu, Xia;Deng, Ping

作者机构：

关键词： Streptococcus iniae; Largemouth bass; Whole genome; Virulence; Drug resistance

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 40 卷

页码：

收录情况： SCI

摘要： To investigate the cause of mass mortality in largemouth bass (Micropterus salmoides) at an aquaculture farm in Hubei due to acute hemorrhagic disease, a dominant bacterium named LY02 was isolated from the brain and major internal organs of diseased fishdise. A re-infection experiment confirmed that LY02 was the pathogenic bacterium responsible for the mass mortality. Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes simA, pdi, sagA, and cfi. Whole-genome analysis identified LY02 as Streptococcus iniae, carrying 330 toxin factor-related genes and 164 resistance factor-related genes. LY02 was found to be highly sensitive to ceftizoxime, cefradine, cefotaxime, azithromycin, erythromycin, enoxacin, doxycycline, and netilmicin; moderately sensitive to norfloxacin and enrofloxacin; and resistant to gentamicin, streptomycin, kanamycin, tobramycin, and neomycin. This study is the first to isolate and identify S. iniae from infected largemouth bass, providing a theoretical basis for the diagnosis and prevention of acute hemorrhagic disease in this species.

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