Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis

文献类型： 外文期刊

第一作者： Wang, Zhenhui

作者： Wang, Zhenhui;Li, Mingxia;Kong, Ziyi;Wang, Endong;Zhang, Bo;Lv, Jiale;Xu, Xuenong

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关键词： Phytoseiidae; nanomaterial; soaking; reproduction

期刊名称：NANOMATERIALS （ 影响因子：5.719； 五年影响因子：5.81 ）

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年卷期： 2022 年 12 卷 21 期

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收录情况： SCI

摘要： RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of P. persimilis (PpATPb, PpATPd, PpRpL11, PpRpS2, and Pptra-2) were selected to verify whether SPc promotes the delivery of dsRNA into P. persimilis through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When PpATPb, PpATPd, PpRpL11, or PpRpS2 was interfered, both the fecundity and egg hatching rate decreased. In contrast, when Pptra-2 was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, P. persimilis expression of PpRpL11 reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites.

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