Functional characterization of differentially expressed proteins coming from unisexual and bisexual infected Schistosoma japonicum female worms

文献类型： 外文期刊

第一作者： Zhong, Haoran

作者： Zhong, Haoran;Qin, Fanglin;Ren, Yuqi;Li, Xiaochun;Hou, Ling;Jin, Yamei;Zhong, Haoran;Qin, Fanglin;Ren, Yuqi;Li, Xiaochun;Hou, Ling;Jin, Yamei;Qin, Fanglin;Li, Xiaochun;Hou, Ling;Gu, Shaopeng

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关键词： Schistosoma japonicum; Differentially expressed protein; Biological functions; Gene knockdown; Immunization

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.1； 五年影响因子：2.1 ）

ISSN： 0014-4894

年卷期： 2023 年 248 卷

页码：

收录情况： SCI

摘要： Schistosomiasis is an important zoonotic disease affecting up to 40 kinds of animals and is responsible for similar to 250 million human cases per year. Due to the extensive use of praziquantel for the treatment of parasitic diseases, drug resistance has been reported. Consequently, novel drugs and effective vaccines are urgently needed for sustained control of schistosomiasis. Targeting reproductive development of Schistosoma japonicum could contribute to the control of schistosomiasis. In this study, five highly expressed proteins (S. japonicum large subunit ribosomal protein L7e, S. japonicum glutathione S-transferase class-mu 26 kDa isozyme, S. japonicum UDP-galactose-4-epimerase and two hypothetical proteins SjCAX70849 and SjCAX72486) in 18, 21, 23, and 25-day mature female worms compared to single-sex infected female worms were selected based on our previous proteomic analysis. Quantitative real-time polymerase chain reaction analysis and long-term interference with small interfering RNA were performed to identify the biological functions of these five proteins. The transcriptional profiles suggested that all five proteins participated in the maturation of S. japonicum. RNA interference against these proteins resulted in morphological changes to S. japonicum. The results of an immunoprotection assay revealed that immunization of mice with recombinant SjUL-30 and SjCAX72486 upregulated production of immunoglobulin G-specific antibodies. Collectively, the results demonstrated that these five differentially expressed proteins were vital to reproduction of S. japonicum and, thus, are potential candidate antigens for immune protection against schistosomiasis.

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