ZIF-8@GOx@PtNPs driven three-mode portable biosensor: smartphone-assisted sensitive detection of EHEC O157:H7 in food samples

文献类型： 外文期刊

第一作者： Wan, Yue

作者： Wan, Yue;Wang, Jiahong;Han, Tingting;Shi, Yueru;Zhang, Wenhui;Wan, Yue;Wang, Jiahong;Sun, He;Bu, Shengjun;Han, Tingting;Shi, Yueru;Zhang, Zebin;Li, Nan;Hao, Zhuo;Wan, Jiayu;Ma, Chengyou

作者机构：

关键词： Enterohemorrhagic Escherichia coli O157:H7; Nanozyme; Three-mode detection; Smartphone assisted detection; Portable device

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2025 年 192 卷 9 期

页码：

收录情况： SCI

摘要： Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) is an important zoonotic pathogen threatening global food safety. The development of rapid and sensitive on-site detection technologies is of great significance for preventing foodborne disease outbreaks. In this study, a tri-mode portable biosensor integrating magnetic separation (Fe3O4), nanozyme catalysis (ZIF-8@GOx@PtNPs), and smartphone assisted detection was constructed for the point-of-care detection of EHEC O157:H7. By simultaneously loading glucose oxidase (GOx) and platinum nanoparticles (PtNPs) on the ZIF-8 scaffold, its dual catalytic activities were utilized: GOx catalyzes the oxidation of glucose to generate H2O2; PtNPs exhibit peroxidase-like activity to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2. Three signals were generated to trigger three detection modes: i. glucose consumption signal read by a smartphone blood glucose meter; ii. photothermal signal monitored by a smartphone infrared thermometer; and iii. colorimetric signal captured by a smartphone camera. The system achieved a detection limit of 9 CFU/mL within 50 min, with a linear range of 10(1) - 10(9) CFU/mL. The tri-mode signals verified each other, significantly improving detection reliability, and the recoveries for milk, meat, and orange juice were 92.7% - 108.6%. This biosensor enables on-site multi-modal detection of EHEC O157:H7, providing an innovative technical approach for food processing supervision, water source safety assessment, and livestock health monitoring. Its modular design can be extended to the detection of other foodborne pathogens.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序