文献类型： 外文期刊
第一作者： Wei, Jiaqi
作者： Wei, Jiaqi;Wang, Chunye;Zhang, Tianyu;Qu, Guijuan;Wei, Jiaqi;Zhang, Tianyu;Hao, Zhuo;Wan, Jiayu
作者机构：
关键词： Hybrid nanocomposites, electrochemical biosensor, LFIA; E.Coli O157:H7; Visual detection
期刊名称：BIOELECTROCHEMISTRY （ 影响因子：4.5； 五年影响因子：4.4 ）
ISSN： 1567-5394
年卷期： 2026 年 167 卷
页码：
收录情况： SCI
摘要： Prevention and control of foodborne pathogens are essential for public health safety. This study integrates organic-inorganic hybrid nanocomposites, lateral flow immunochromatography, and screen-printed electrodes to develop an innovative electrochemical biosensor for detecting foodborne pathogens. Two types of organic-inorganic hybrid nanocomposites incorporated onto a conjugate pad or nitrocellulose membrane support were used. In the presence of foodborne pathogens, a sandwich immune complex structure forms on the detection line. Ferrocene within the sandwich structure is detected using differential pulse voltammetry. The biosensor specifically detects foodborne pathogens with a minimum detection limit of 25 CFU center dot mL(-1) and a detection linear range of 10(2) to 10(8) CFU center dot mL(-1), demonstrating excellent specificity and sensitivity. This biosensor can be integrated with smartphones, enhancing the intelligence and miniaturization of biosensors.
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