Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA

文献类型： 外文期刊

第一作者： Wang, Lingling

作者： Wang, Lingling;Zhang, Xiaoying;Su, Yuchun;Bu, Shengjun;Meng, Heyuan;Zhou, Hongyu;Hao, Zhuo;Wan, Jiayu;Bu, Shengjun;Xu, Chuanna

作者机构：

关键词： Influenza virus; M13mp18 ssDNA; Lateral flow assay; Isothermal amplification

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2025 年 192 卷 3 期

页码：

收录情况： SCI

摘要： A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10-106 pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序