Characterization of a broad-spectrum endolysin LysSP1 encoded by a Salmonella bacteriophage

文献类型： 外文期刊

第一作者： Jiang, Yanhua

作者： Jiang, Yanhua;Xu, Dongqin;Wang, Lianzhu;Qu, Meng;Li, Fengling;Tan, Zhijun;Yao, Lin;Xu, Dongqin;Tan, Zhijun

作者机构：

关键词： Bacteriophage; Endolysin; Salmonella; Bactericidal activity

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2021 年 105 卷 13 期

页码：

收录情况： SCI

摘要： Foodborne pathogens have caused many public health incidents and heavy economic burden. Endolysins have been proven to have efficient bactericidal activity against pathogens with low incidence of resistance. In this study, the recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained by prokaryotic expression, and its characteristics were analyzed. Ethylenediaminetetraacetic acid (EDTA) can be used as the outer membrane permeabilizer to increase the bactericidal activity of LysSP1. Under the synergism of 5 mmol/L EDTA, LysSP1 exhibited a strong bactericidal activity against Salmonella Typhimurium ATCC14028. LysSP1 was stable at 4 degrees C for 7 days and at -20 degrees C for 180 days. LysSP1 remained the optimal activity at 40 degrees C and was efficiently active at alkaline condition (pH 8.0-10.0). Divalent metal ions could not enhance the bactericidal activity of LysSP1 and even caused the significant reduction of bactericidal activity. LysSP1 not only could lyse Salmonella, but also could lyse other Gram-negative strains and Gram-positive strains. These results indicated that LysSP1 is a broad-spectrum endolysin and has potential as an antimicrobial agent against Salmonella and other foodborne pathogens.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序