Structural elucidation and immuno-stimulating activity of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba

文献类型： 外文期刊

第一作者： Du, XiuJu

作者： Du, XiuJu;Zhang, JingSong;Yang, Yan;Tang, QingJiu;Jia, Wei;Liu, YanFang;Zhou, Shuai;Hao, Ruixia;Du, XiuJu;Ye, LiBin;Gong, ChunYu;Pan, YingJie;Du, XiuJu

作者机构：

关键词： Tremella aurantialba;Polysaccharide;Structural elucidation;NMR spectroscopy;lmmuno-stimulating activity

期刊名称：CARBOHYDRATE RESEARCH （ 影响因子：2.104； 五年影响因子：2.192 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A novel acidic heteropolysaccharide (TAPA1) was purified from hot water extracts of Tremella aurantialba fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-500 High-Resolution Chromatography. The heteropolysaccharide had a molecular weight of ca. 1.35 x 106 Da, and a carbohydrate content estimated to be -98.7% by the phenol-sulfuric acid method. It was composed mainly of o-mannose, o-xylose, and D_glucuronic acid in the ratio of ca. 5:4:1, along with trace amounts of D-galacturonic acid and o-glucose. Monosaccharide compositional analysis and GC-MS of methylated derivatives, combined with 1H and 13C NMR spectroscopies (including COSY, TOCSY, NOESY, HSQC, and HMBC spectra), revealed TAPA1 to consist of an α-(1_ú3)-linked mannopyranosyl backbone, partially substituted at position 4 with xylose side chains, and at position 2 with side chains composed of either xylose, mannose, and glucuronic acid or of xylose and mannose. Bioactivity testing showed that TAPA1 stimulated the proliferation of mouse spleen lymphocytes in vitro.

分类号： O62

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